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Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation

Award Number: R44AR073136
ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/07/2017
PERIOD OF PERFORMANCE END DATE: 08/31/2023

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Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation - Resurfacing of articular cartilage with cold stored osteochondral allografts is employed clinically for repair of trauma and osteoarthritis-induced articular cartilage surface damage. Chondrocyte viability of transplanted articular cartilage is accepted as one of the determinants of outcome following osteochondral allograft transplantation. We have previously developed an ice-free vitrification method of cryopreservation that maintains excellent chondrocyte viability in animal model and human articular cartilage. The chondrocytes survive vitrification due to the absence of ice formation during cooling and warming of 1-3mL samples. However, it had not been possible to rewarm larger samples due to ice nucleation during rewarming that results in loss of chondrocyte viability prior to our Phase I studies. The innovation in this proposal relates to a new rewarming method that does not have the limitations of boundary convection warming that should be effective for samples up to 50mL in volume. This rewarming method utilizes radio frequency induced heating of magnetic iron nanoparticles. In Phase I we demonstrated the effectiveness of ice-free vitrification combined with nanowarming for large full thickness osteochondral tissues in 50 mL volumes in 80 seconds with maintenance of both chondrocyte viability and extracellular matrix integrity. In Phase II we propose development of a porcine model, further optimization of ice-free vitrification and nanowarming and in vivo evaluation in a porcine model in three specific aims. Nanowarmed chondrocyte viability, chemistry, and biomaterial properties will be compared with untreated fresh control tissues both before and after transplant. The nanowarming conditions that provides the best preservation of chondrocytes with minimal if any cartilage biomaterial changes will be selected for further investigation and translation to human cartilage in a subsequent Phase IIb SBIR application.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = -$91,818 )
2024	2020	TISSUE TESTING TECHNOLOGIES LLC	2231 TECHNICAL PKWY	CHARLESTON	SC	29406	CHARLESTON	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	3	3/17/2024	NON-COMPETING CONTINUATION	-$91,818
														Subtotal = -$91,818

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2020	TISSUE TESTING TECHNOLOGIES LLC	2231 TECHNICAL PKWY	CHARLESTON	SC	29406	CHARLESTON	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	3	9/16/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $826,749 )
2020	2020	TISSUE TESTING TECHNOLOGIES LLC	2231 TECHNICAL PKWY STE A	NORTH CHARLESTON	SC	29406	CHARLESTON	USA	Arthritis, Musculoskeletal and Skin Diseases Research	001	3	8/25/2020	NON-COMPETING CONTINUATION	$826,749
2020	2019	TISSUE TESTING TECHNOLOGIES LLC	2231 TECHNICAL PKWY STE A	NORTH CHARLESTON	SC	29406	CHARLESTON	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	2	2/27/2020	COMPETING CONTINUATION	$0
														Subtotal = $826,749

Issue Date FY: 2019 ( Subtotal = $838,363 )
2019	2019	TISSUE TESTING TECHNOLOGIES LLC	2231 TECHNICAL PKWY STE A	NORTH CHARLESTON	SC	29406	CHARLESTON	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	2	9/11/2019	COMPETING CONTINUATION	$838,363
														Subtotal = $838,363

Grand Total All Awards = $1,573,294

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Ice-free vitrification and nanowarming of large osteochondral grafts for transplantation

Award Number: R44AR073136

ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/07/2017

PERIOD OF PERFORMANCE END DATE: 08/31/2023

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