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Rapid, Simple and Cost-Effective Detection of Human Cell Line Contamination

Award Number: R44AG061957
ORGANIZATION: NATIONAL INSTITUTE ON AGING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2018
PERIOD OF PERFORMANCE END DATE: 08/31/2019

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Rapid, Simple and Cost-Effective Detection of Human Cell Line Contamination - Summary Cell line contamination and misidentification have dramatic repercussions in the field biomedical research, largely contributing to the growing concerns about irreproducible results and posing a significant burden on the global biomedical research budget. While the general awareness about these issues has raised over the last years, current authentication technologies such as short tandem repeat (STR) profiling are often labor intensive, slow, costly and not suited for cancer cell lines, which make it very difficult to reliably and continuously monitor human cell cultures. Profiling single nucleotide polymorphisms (SNPs) would provide a good alternative to STR for monitoring cancer cell cultures, SNPs being less affected by genomic instabilities than STR markers. NuProbe’s blocker displacement amplification (BDA) technology uniquely enables multiplexed rare allele enrichment by PCR. Building upon NuProbe’s BDA technology, we propose to develop a series of highly multiplexed and highly sensitive qPCR-based SNP profiling assays to detect intra-species cross-contaminants present in human cell cultures at low levels (down to 1%). We anticipate these assays to translate into rapid, cost effective, reliable, and easy-to-use cell line contamination detection kits compatible with commonly available qPCR instruments.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = -$26,854 )
2023	2018	NUPROBE USA INC	2575 W BELLFORT AVE	HOUSTON	TX	77054	HARRIS	USA	Aging Research	000	1	3/9/2023	NEW	-$26,854
														Subtotal = -$26,854

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2020	NUPROBE USA, INC.	2575 W BELLFORT AVE STE 200	HOUSTON	TX	77054	HARRIS	USA	Aging Research	001	3	7/26/2021	NON-COMPETING CONTINUATION	$0
2021	2018	NUPROBE USA, INC.	2575 W BELLFORT AVE STE 200	HOUSTON	TX	77054	HARRIS	USA	Aging Research	000	1	7/21/2021	NEW	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $254,960 )
2020	2020	NUPROBE USA, INC.	85 BOLTON ST	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Aging Research	001	3	8/21/2020	NON-COMPETING CONTINUATION	$254,960
2020	2019	NUPROBE USA, INC.	85 BOLTON ST	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Aging Research	000	2	11/6/2019	EXTENSION WITH OR WITHOUT FUNDS	$0
														Subtotal = $254,960

Issue Date FY: 2019 ( Subtotal = $716,643 )
2019	2019	NUPROBE USA, INC.	85 BOLTON ST	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Aging Research	001	2	8/29/2019	EXTENSION WITH OR WITHOUT FUNDS	$716,643
2019	2018	NUPROBE USA, INC.	85 BOLTON ST	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Aging Research	000	1	1/9/2019	NEW	$0
														Subtotal = $716,643

Issue Date FY: 2018 ( Subtotal = $749,300 )
2018	2018	NUPROBE USA, INC.	85 BOLTON ST	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Aging Research	000	1	9/17/2018	NEW	$749,300
														Subtotal = $749,300

Grand Total All Awards = $1,694,049

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Rapid, Simple and Cost-Effective Detection of Human Cell Line Contamination

Award Number: R44AG061957

ORGANIZATION: NATIONAL INSTITUTE ON AGING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2018

PERIOD OF PERFORMANCE END DATE: 08/31/2019

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