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Same day embryo viability sensor for high throughput embryo transfer selection

Award Number: R43OD030028
ORGANIZATION: OFFICE OF THE DIRECTOR, NIH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2021
PERIOD OF PERFORMANCE END DATE: 12/31/2022

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Same day embryo viability sensor for high throughput embryo transfer selection - Abstract The biomedical research community extensively relies on the availability and continued utilization of the rodent research model. With the discovery of CRISPR, and the unprecedented degree with which it allowed scientists to manipulate the genome, an explosion of clinically relevant models has made their way into existence. This leading edge of precision medicine is responsible for the phenotyping of harmful SNP mutations, modeling of disease variants in ethnically distinct populations, and understanding of orphan diseases. Production of these models heavily relies upon in-vitro assisted reproductive techniques including cryo- preservation, gene editing, and in-vitro fertilization however these techniques also reduce the reproductive viability of embryos and the percentage of live-born. As the number of unique models continues to increase facilities require automated and standardized in-vitro assisted reproductive tools to flexibly scale with demand and maintain larger litters. To date embryo handling has been done via mouth pipetting, and embryo viability has been assessed through attrition by either transferring all the available embryos as soon as possible or waiting several days for the damaged cells to eventually arrest leaving some competent blastocysts. Recent developments have measured oocyte/embryo mechanical properties as a non-invasive bio- marker of viability by optically measuring embryo deformation when aspirating through a micro-pipette; however, this technique is still manually demanding and measurement collection systems are imperfect. We propose building a high-throughput microfluidic lab on chip that can identify the viability of embryos immediately after exposure to environmental stresses brought on from cryo-preservation, gene editing, and in-vitro fertilization. Building on the known predictive power of zygote mechanical properties and Ravata’s micro-electrode platform, we would validate an electrical sensor array to electrically quantify embryo displacement through a microfluidic aspirator to measure nuclear and cytoplasmic maturation as an early embryo viability detection system.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	RAVATA SOLUTIONS INC	630 PENA DR	DAVIS	CA	95618	YOLO	USA	Research Infrastructure Programs	000	1	10/25/2023	NEW	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $144,560 )
2021	2021	RAVATA SOLUTIONS, INC.	2020 5TH ST #396	DAVIS	CA	95617	YOLO	USA	Research Infrastructure Programs	000	1	6/29/2021	NEW	$144,560
														Subtotal = $144,560

Grand Total All Awards = $144,560

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Same day embryo viability sensor for high throughput embryo transfer selection

Award Number: R43OD030028

ORGANIZATION: OFFICE OF THE DIRECTOR, NIH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2021

PERIOD OF PERFORMANCE END DATE: 12/31/2022

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