Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Multiplexing methods to reduce sequencing costs.

Award Number: R43HG010445
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2019
PERIOD OF PERFORMANCE END DATE: 08/31/2020

Group Awards By:

View Award Description

Multiplexing methods to reduce sequencing costs. - Project Summary As the capacity of next generation sequencing machines has increased, the marginal cost of shallow sequencing has become much higher than that of deep sequencing. For the development of novel protocols, quality checking, and targeted clinical sequencing, sequencing demand is often far less than an entire sequencing run. This issue can be resolved if a user can find a partner performing a high depth sequencing run to “spike” their library into. By pooling samples together, both users can save considerably on sequencing costs. However, this process of finding sequencing partners is subject to local availability of sequencing supply and demand. Moreover, variation in representation due to user error and library specific biases can reduce the efficiency of the pooling. An integrated platform for sample pooling called WeSeq is proposed. The primary goal of WeSeq is to maximize the time and cost efficiency of next generation sequencing. WeSeq develops algorithms to quickly turnaround sequencing analysis results to end users and to minimize the representation biases associated with pooling large numbers of diverse libraries together. We develop enzymatic approaches for greatly increasing DNA barcoding capacity and for reducing the number of sequencing required for applications such as single cell RNA-seq. Ultimately, we hope ot make next-generation sequencing affordable and fast enough for it to become routine in research and clinical applications.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2019	CORAL GENOMICS, INC.	953 INDIANA ST	SAN FRANCISCO	CA	94107	SAN FRANCISCO	USA	Human Genome Research	000	1	2/10/2021	NEW	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $142,674 )
2019	2019	CORAL GENOMICS, INC.	953 INDIANA ST	SAN FRANCISCO	CA	94107	SAN FRANCISCO	USA	Human Genome Research	000	1	8/30/2019	NEW	$142,674
														Subtotal = $142,674

Grand Total All Awards = $142,674

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Multiplexing methods to reduce sequencing costs.

Award Number: R43HG010445

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2019

PERIOD OF PERFORMANCE END DATE: 08/31/2020

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer