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Affinity reagents for the detection of modified RNAs and Transcriptome-wide mapping of RNA modifications

Award Number: R43DA038991
ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2014
PERIOD OF PERFORMANCE END DATE: 08/31/2016

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Affinity reagents for the detection of modified RNAs and Transcriptome-wide mapping of RNA modifications - DESCRIPTION (provided by applicant): Biological RNAs undergo extensive post-transcriptional modifications. These modifications are important in promoting the accuracy and efficiency of gene expression in biological systems. To decipher the functions of RNA modifications, robust analytical tools and methods that enable scientists to monitor, manipulate, and transcriptome-wide mapping RNA modifications are highly needed. In this multi-phase SBIR program, Guild Biosciences Inc. (GBS) proposes to address this issue by: 1) developing a library of affinity antibodies and peptide sequences specific to modified nucleosides by using phase-antibody display libraries and peptide phase-display library, 2) developing a novel immunoassay system- open immunocomplex assay with the developed antibodies and peptides for rapid and quantitative detection of modified nucleotides in RNA samples, and 3) incorporating the immunoprecipitation capability of the developed affinity reagents with next-generation sequencing platforms to develop method for global mapping of RNA modifications with high accuracy. Successful completion of the proposed research will add much needed analytical tools and methods in terms of affinity reagents, immunoassay systems and deep sequencing method, which will significantly expand the tool box for the epigenetic research. Several commercial viable products, such as affinity reagents (antibodies, peptides) recognizes the chemical structures of modified RNA nucleosides, bioassay kits for detection/quantitation of RNA modifications, and sequencing preparation kit for deep RNA sequencing will be generated as the ultimate outcome of this proposed research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2020 ( Subtotal = -$748 )
2020	2014	GUILD ASSOCIATES, INC.	5750 SHIER RINGS RD	DUBLIN	OH	43016	FRANKLIN	USA	Drug Use and Addiction Research Programs	000	1	10/1/2019	NEW	-$748
														Subtotal = -$748

Issue Date FY: 2014 ( Subtotal = $209,008 )
2014	2014	GUILD ASSOCIATES INC	5750 SHIER RINGS	DUBLIN	OH	43016	FRANKLIN	USA	Drug Use and Addiction Research Programs	000	1	8/13/2014	NEW	$209,008
														Subtotal = $209,008

Grand Total All Awards = $208,260

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Affinity reagents for the detection of modified RNAs and Transcriptome-wide mapping of RNA modifications

Award Number: R43DA038991

ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2014

PERIOD OF PERFORMANCE END DATE: 08/31/2016

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