Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Development of lavage EV protein biomarkers for minimally-invasive detection of endometrial cancer

Award Number: R43CA272045
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2022
PERIOD OF PERFORMANCE END DATE: 08/31/2024

Group Awards By:

View Award Description

Development of lavage EV protein biomarkers for minimally-invasive detection of endometrial cancer - PROJECT SUMMARY Early detection of endometrial cancer can increase the 5-year survival rate from <20% to >95%. However, currently there are no non-invasive methods for its detection. We propose the use of cell-secreted extracellular vesicles (EVs) as the source of new diagnostic biomarkers from uterine lavage – a sample that is much more targeted to endometrial cancer. Within the scope of biomarker detection, EVs offer numerous benefits for clinical analysis, including non-invasive collection, a suitable sample source for longitudinal disease monitoring, higher stability and sample volumes, faster processing times and lower cost. However, the analysis of exosomes and other EV has not really been possible for early endometrial cancer detection. The common procedures for biofluid EV biomarker analysis are very long and cumbersome due to extremely low target levels and high background of free proteins. In order to enable better biomarker discovery and endometrial cancer diagnosis, a more reliable and efficient approach is needed, capable of enriching potential cancer-associated proteins with higher purity. In this NIH SBIR Phase I study, we will implement our novel method for fast and reproducible enrichment of EVs with >95% recovery yield and >99.9% purity for analysis of endometrial cancer from lavage samples. We have already carried out initial lavage EV validation and biomarker discovery experiments, and selected over a dozen of high-quality protein targets that enable effective differentiation of endometrial cancer from non-cancer controls. The following aims will be completed in the Phase I of the proposal: Aim #1: Determine the feasibility of the discovered endometrial cancer biomarkers from lavage EVs to differentiate the disease and refine the panel. Aim #2: Evaluate and implement the novel uterine lavage collection device for fast and effective EV isolation and measurement. By the completion of this project, the non-invasive endometrial cancer biomarker discovery platform from lavage EVs will be developed, and a minimally invasive early endometrial cancer detection assay will be validated that can overcome the limitations of current approaches, and thus could have an enormous public health impact and market potential.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2022	TYMORA ANALYTICAL OPERATIONS LLC	1201 CUMBERLAND AVE	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Cancer Detection and Diagnosis Research	000	1	1/8/2025	NEW	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $290,000 )
2022	2022	TYMORA ANALYTICAL OPERATIONS, LLC	1201 CUMBERLAND AVE STE 209	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Cancer Detection and Diagnosis Research	000	1	8/19/2022	NEW	$290,000
														Subtotal = $290,000

Grand Total All Awards = $290,000

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Development of lavage EV protein biomarkers for minimally-invasive detection of endometrial cancer

Award Number: R43CA272045

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2022

PERIOD OF PERFORMANCE END DATE: 08/31/2024

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer