Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Rapid sample preparation method for high molecular weight DNA from tumor tissues suitable for structural variant analysis

Award Number: R43CA239967
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/03/2019
PERIOD OF PERFORMANCE END DATE: 04/02/2020

Group Awards By:

View Award Description

Rapid sample preparation method for high molecular weight DNA from tumor tissues suitable for structural variant analysis - Summary Claremont BioSolutions, LLC (CBIO) The introduction of long-read sequencing and mapping applications is set to change the field of cancer research by allowing unparalleled insight into the complex structure of tumor genomes. With the ability to sequence large spans of DNA that are 10s-100s of kb in size these technologies will offer more accurate de novo assembly, resolve highly repetitive regions, and allow identification of large structural variants in tumor genomes. As cancer researchers move to these platforms they will face new hurdles as effective sample preparation of ultra-long DNA (>250 kb) remains a limiting step. Current methods of DNA extraction, including spin column and magnetic bead applications, significantly shear ultra-long DNA. Older, albeit effective, agarose plug digestion techniques are difficult, time-consuming and highly variable. New commercial products have been introduced that can isolate ultra-long DNA but are restricted in use to bacteria, tissue culture cells, or fresh blood cells, and are not usable with clinically relevant solid tumor tissue samples. To overcome this challenge, Claremont BioSolutions is developing a prototype technology that combines gentle and rapid tissue dissociation with a novel binding matrix to isolate high quality/high quantity ultra-long DNA in <20 minutes. The method reduces DNA shearing and tangling and is compatible with small quantities of solid tissue. In Aim 1, we shall use the combined approach to demonstrate isolation of ultra-long DNA (>250 kb) from matched normal and tumor tissue samples and analyze length and quality. In Aim 2, we shall validate compatibility of the isolated ultra-long DNA with third generation long-read sequencing and mapping technologies and analyze the sequence data to demonstrate improved long-read sequencing over existing DNA extraction methods. Ultimately, the goal will be to integrate this miniature technology into an automated platform to provide a rapid, cost effective, and reproducible method to isolate DNA from solid tissues for improved downstream analysis of tumor samples.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2019	CLAREMONT BIOSOLUTIONS LLC	1182 MONTE VISTA AVE 11	UPLAND	CA	91786	SAN BERNARDINO	USA	Cancer Cause and Prevention Research	000	1	10/13/2020	NEW	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $299,988 )
2019	2019	CLAREMONT BIOSOLUTIONS LLC	1182 MONTE VISTA AVE 11	UPLAND	CA	91786	SAN BERNARDINO	USA	Cancer Cause and Prevention Research	000	1	4/3/2019	NEW	$299,988
														Subtotal = $299,988

Grand Total All Awards = $299,988

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Rapid sample preparation method for high molecular weight DNA from tumor tissues suitable for structural variant analysis

Award Number: R43CA239967

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/03/2019

PERIOD OF PERFORMANCE END DATE: 04/02/2020

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer