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FLASH-pulse: A versatile multiplexed tissue imaging solution for research on aging

Award Number: R43AG092073
ORGANIZATION: NATIONAL INSTITUTE ON AGING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/22/2025
PERIOD OF PERFORMANCE END DATE: 09/21/2027

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FLASH-pulse: A versatile multiplexed tissue imaging solution for research on aging - Project Summary Addressing the complexities of age-related disorders at the cellular and molecular level requires a spatial omics technology capable of rapidly and reliably mapping the distribution of specific cell markers within tissue at the subcellular scale. While spatial proteomics technologies have revolutionized in situ molecular profiling of tissues, limitations in resolution, throughput, sample degradation, and cost have hindered their widespread impact. This is the focus of our project, which proposes to develop FLASH-PAINT, a disruptive proteomics technology, into FLASH-pulse, an automated and multiplexed imaging solution specifically tailored for FFPE tissue samples. Our proposed technology promises to provide scientists with a simple method to perform exceptionally rapid 32-plex protein imaging on their tissue samples using a microfluidics chamber designed for easy integration with standard benchtop microscopes and necessitating minimal experimental intervention on their part. FLASH-pulse is distinguished by its elimination of washing steps, thereby preventing sample degradation and enabling the rapid collection of highly multiplexed imaging data of large tissue sections1 cm2 tissue. Panluminate, Inc. is a leading company in the spatial proteomics space. Our proposed project aims to develop FLASH-pulse and validate it in human FFPE tissue. We specifically propose to (1) develop a new concept and prototype for microfluidics tailored for FLASH samples that removes the need of traditional big-footprint microfluidics, (2) develop new FLASH probes designed for high signal-to-background imaging in FFPE tissue, and (3) test this system in a testbed application of aged and young human lymphoid tissue. Ultimately, the developments proposed here will enable panluminate to disseminate FLASH-pulse imaging to every research and biotech lab around the world.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	PANLUMINATE INC	101 COLLEGE STREET, SUITE 210	NEW HAVEN	CT	06510	SOUTH CENTRAL CT	USA	Aging Research	000	1	9/21/2026	NEW	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $397,902 )
2025	2025	PANLUMINATE INC	101 COLLEGE STREET, SUITE 210	NEW HAVEN	CT	06510	SOUTH CENTRAL CT	USA	Aging Research	000	1	9/19/2025	NEW	$397,902
														Subtotal = $397,902

Grand Total All Awards = $397,902

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FLASH-pulse: A versatile multiplexed tissue imaging solution for research on aging

Award Number: R43AG092073

ORGANIZATION: NATIONAL INSTITUTE ON AGING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/22/2025

PERIOD OF PERFORMANCE END DATE: 09/21/2027

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