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Novel assays for assessing protein-protein interactions in Alzheimer's disease

Award Number: R43AG055250
ORGANIZATION: NATIONAL INSTITUTE ON AGING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2016
PERIOD OF PERFORMANCE END DATE: 08/31/2018

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Novel assays for assessing protein-protein interactions in Alzheimer's disease - Abstract Cell-based assays more closely mimic biology than direct inhibition studies. Therefore, drug discovery companies are increasingly utilizing cellular screening assays. Current reporter systems are unable to reliably measure in-cell transient protein-protein interactions. Furthermore, many are based upon split enzyme systems, which require long incubation steps, are not real-time and are typically low throughput. In order to improve on these existing technologies, we will develop a split SUMOstar system engineered to contain a tetracysteine recognition motif for a biarsenical-based fluorophores. There are several advantages for using split a SUMOstar fluorescent reporter system, including relatively small fusions (~6 Kda) to the target proteins of interest, lower non-specific interaction with native proteins and the ability to take real-time measurements in high-throughput applications. Furthermore, using structural based mutagenesis, we can engineer the kinetics and binding affinity of the split SUMO interaction, allowing us to fine-tune the reporter assay to measure transient protein-protein interactions.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2016	LIFESENSORS, INC.	271 GREAT VALLEY PKWY	MALVERN	PA	19355	CHESTER	USA	Aging Research	000	1	10/25/2018	NEW	$0
														Subtotal = $0

Issue Date FY: 2016 ( Subtotal = $224,674 )
2016	2016	LIFESENSORS INC	271 GREAT VALLEY PARKWAY	MALVERN	PA	19355	CHESTER	USA	Aging Research	000	1	9/15/2016	NEW	$224,674
														Subtotal = $224,674

Grand Total All Awards = $224,674

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Novel assays for assessing protein-protein interactions in Alzheimer's disease

Award Number: R43AG055250

ORGANIZATION: NATIONAL INSTITUTE ON AGING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2016

PERIOD OF PERFORMANCE END DATE: 08/31/2018

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