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ULTRASOUND-ACCELERATED TISSUE FORMALIN FIXATION AND PARAFFIN EMBEDDING

Award Number: R33CA091166
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2001
PERIOD OF PERFORMANCE END DATE: 07/31/2011

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ULTRASOUND-ACCELERATED TISSUE FORMALIN FIXATION AND PARAFFIN EMBEDDING - DESCRIPTION (provided by applicant): The first crucial step in cancer management is to assure timely and accurate pathological diagnoses. Formalin fixation and paraffin embedding (FFPE) has been a standard tissue preservation method employed in over 90% cases for clinical histology diagnosis. Though it provides superior morphology and easy long- term storage of clinical specimens, FFPE is time-consuming and does not fully support current molecular analyses. The long-term goal of our research is to apply modern techniques to medical practice and pathological diagnosis to effectively fight cancers and other diseases. Our proposed project is to further develop the ultrasound-accelerated tissue preservation (UTP) technology for multiple tissues and to study the mechanism of ultrasound (US)-facilitated formalin fixation. Our specific aims are: (1) to develop a multi- tissue preservation processor with an optional real-time digital system to monitor and standardize tissue fixation level; (2)to validate the UTP techniques by performing more statistical assessments on histopathology, macromolecule integrity, and their long-term stability; and (3) to conduct mechanism studies to elucidate the effects of formalin fixation with and without US on the formation of cross-linking, enzymatic activity, and protein conformational changes. We have demonstrated that in comparison to conventional FFPE, UTP provides similar preservation in tissue morphology with similar long-term storage stability, improved preservation of protein structure, antigen properties, and mRNA integrity. UTP allows easy general molecular profiling and analyses based on extracts from UTP-fixed tissues. UTP also provides a good opportunity to control and monitor fixation level by adjusting the time and strength of the ultrasound. We hypothesize that US-facilitated formalin fixation will greatly accelerate formaldehyde-induced macromolecule cross-linking in tissues and "freeze" macromolecules and their conformation due to accelerated fixation reactions. Since tissue preservation is still a standard and general requirement before histology diagnosis, the innovation should have great impact in economy and public health.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2010 ( Subtotal = $0 )
2010	2008	AMERICAN REGISTRY OF PATHOLOGY	14TH ST & ALASKA AVE NW #54	WASHINGTON	DC	20306	DISTRICT OF COLUMBIA	USA	Cancer Detection and Diagnosis Research	000	5	6/21/2010	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2008 ( Subtotal = $206,226 )
2008	2008	AMERICAN REGISTRY OF PATHOLOGY	14TH ST & ALASKA AVE NW #54	WASHINGTON	DC	20306	DISTRICT OF COLUMBIA	USA	Cancer Detection and Diagnosis Research	000	5	7/9/2008	NON-COMPETING CONTINUATION	$206,226
														Subtotal = $206,226

Issue Date FY: 2007 ( Subtotal = $204,117 )
2007	2007	AMERICAN REGISTRY OF PATHOLOGY	14TH ST & ALASKA AVE NW #54	WASHINGTON	DC	20306	DISTRICT OF COLUMBIA	USA	Cancer Detection and Diagnosis Research	000	4	9/6/2007	NON-COMPETING CONTINUATION	$204,117
														Subtotal = $204,117

Issue Date FY: 2006 ( Subtotal = $204,091 )
2006	2006	AMERICAN REGISTRY OF PATHOLOGY	14TH ST & ALASKA AVE NW #54	WASHINGTON	DC	20306	DISTRICT OF COLUMBIA	USA	Cancer Detection and Diagnosis Research	000	3	8/24/2006	COMPETING CONTINUATION	$204,091
														Subtotal = $204,091

Grand Total All Awards = $614,434

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ULTRASOUND-ACCELERATED TISSUE FORMALIN FIXATION AND PARAFFIN EMBEDDING

Award Number: R33CA091166

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2001

PERIOD OF PERFORMANCE END DATE: 07/31/2011

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