Wednesday, August 27, 2025 8/27/2025

CSHL Quantitative Imaging: From Acquisition to Analysis Course

Award Number: R25CA078139
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/1998
PERIOD OF PERFORMANCE END DATE: 06/30/2027

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CSHL Quantitative Imaging: From Acquisition to Analysis Course - Project Description or Abstract Cancer is a highly heterogeneous disease, and the development of successful treatment strategies requires an understanding of the cellular mechanisms that result in diversity of cancer cell dynamics and interactions with the tissue environment. Quantitative imaging is a powerful tool for understanding these cellular mechanisms. New quantitative imaging methods, including light sheet microscopy, super-resolution microscopy and machine learning approaches to biological image analysis, have enabled previously impossible experiments. Proper training in the fundamental principles of quantitative imaging is essential to generating reliable data, correctly interpreting experiments, and enhancing the reproducibility of cancer research. The Cold Spring Harbor Laboratory proposes to continue the course “Quantitative Imaging: From Acquisition to Analysis”, to be held in the spring of 2021-2025. This two-week intensive course provides students with a thorough foundation in state-of-the-art quantitative light microscopy and image analysis techniques. The course is designed to engage a multidisciplinary audience, including biologists, physicists, engineers and computer scientists at all career stages, from graduate student to Principal Investigator. A unique aspect of this course is its strong emphasis on generating accurate and precise quantitative imaging data. A typical day of the course includes didactic lectures, a research seminar, a group discussion session and focused quantitative imaging laboratory exercises. The exercises include both image acquisition and analysis, and are planned such that, over the duration of the course, the most common types of quantitative measurements used in cancer research are covered, including: co-localization, measurement of fluorescence intensities, live specimen imaging,3D-image analysis, and detection/tracking/counting of diffraction-limited objects, subcellular structures, and whole cells. Students learn to use a wide range of imaging modalities to complete the exercises, including widefield fluorescence, point-scanning and spinning disk confocal, total internal reflection fluorescence, light sheet and super-resolution microscopy. A suite of computers equipped with free open-source software are used to process data and make measurements using modern image analysis methods, including machine learning approaches. In the daily group discussion, students present and discuss their results with the course instructors, thereby developing expertise in assessing the quality of quantitative measurements and critical troubleshooting skills. The instructors are internationally recognized leaders in quantitative microscopy and computational image analysis, and invited seminar speakers provide examples of expert use of quantitative imaging to answer biological questions. Quantitative Imaging: From Acquisition to Analysis provides training in quantitative microscopy that is crucial for advancing the mission of the NCI.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $104,193 )
2025	2025	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPG HBR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	26	8/6/2025	NON-COMPETING CONTINUATION	$104,193
														Subtotal = $104,193

Issue Date FY: 2024 ( Subtotal = $104,193 )
2024	2024	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPG HBR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	25	6/21/2024	NON-COMPETING CONTINUATION	$104,193
														Subtotal = $104,193

Issue Date FY: 2023 ( Subtotal = $99,377 )
2023	2023	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	24	6/30/2023	NON-COMPETING CONTINUATION	$99,377
														Subtotal = $99,377

Issue Date FY: 2022 ( Subtotal = $104,193 )
2022	2022	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	002	23	7/15/2022	COMPETING CONTINUATION	$4,816
2022	2022	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	23	7/8/2022	COMPETING CONTINUATION	$104,193
2022	2020	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	001	22	7/15/2022	NON-COMPETING CONTINUATION	-$4,816
														Subtotal = $104,193

Issue Date FY: 2020 ( Subtotal = $102,233 )
2020	2020	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	22	3/27/2020	NON-COMPETING CONTINUATION	$102,233
														Subtotal = $102,233

Issue Date FY: 2019 ( Subtotal = $97,417 )
2019	2019	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	21	3/28/2019	NON-COMPETING CONTINUATION	$97,417
														Subtotal = $97,417

Issue Date FY: 2018 ( Subtotal = $102,233 )
2018	2018	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	20	3/29/2018	NON-COMPETING CONTINUATION	$102,233
														Subtotal = $102,233

Issue Date FY: 2017 ( Subtotal = $102,233 )
2017	2017	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	000	19	3/29/2017	EXTENSION WITH OR WITHOUT FUNDS	$102,233
2017	2016	COLD SPRING HARBOR LABORATORY	1 BUNGTOWN RD	COLD SPRING HARBOR	NY	11724	SUFFOLK	USA	Cancer Research Manpower	001	18	6/15/2017	COMPETING CONTINUATION	$0
														Subtotal = $102,233

Issue Date FY: 2016 ( Subtotal = $102,233 )
2016	2016	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	18	3/30/2016	COMPETING CONTINUATION	$102,233
														Subtotal = $102,233

Issue Date FY: 2004 ( Subtotal = $76,766 )
2004	2004	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	7	7/7/2004	NON-COMPETING CONTINUATION	$76,766
														Subtotal = $76,766

Issue Date FY: 2003 ( Subtotal = $74,531 )
2003	2003	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	6	9/22/2003	COMPETING CONTINUATION	$74,531
														Subtotal = $74,531

Issue Date FY: 2002 ( Subtotal = $74,037 )
2002	2002	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	5	6/10/2002	NON-COMPETING CONTINUATION	$74,037
														Subtotal = $74,037

Issue Date FY: 2001 ( Subtotal = $71,882 )
2001	2001	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	4	6/7/2001	NON-COMPETING CONTINUATION	$71,882
														Subtotal = $71,882

Issue Date FY: 2000 ( Subtotal = $69,789 )
2000	2000	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	3	6/5/2000	NON-COMPETING CONTINUATION	$69,789
														Subtotal = $69,789

Issue Date FY: 1999 ( Subtotal = $67,757 )
1999	1999	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	2	6/14/1999	NON-COMPETING CONTINUATION	$67,757
														Subtotal = $67,757

Issue Date FY: 1998 ( Subtotal = $61,929 )
1998	1998	COLD SPRING HARBOR LABORATORY	BUNGTOWN ROAD	COLD SPRING HRBR	NY	11724		USA	Cancer Research Manpower	000	1	6/26/1998	NEW	$61,929
														Subtotal = $61,929

Grand Total All Awards = $1,414,996

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CSHL Quantitative Imaging: From Acquisition to Analysis Course

Award Number: R25CA078139

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/1998

PERIOD OF PERFORMANCE END DATE: 06/30/2027

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