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Research Resources for Model Amphibians

Award Number: R24OD010435
ORGANIZATION: OFFICE OF THE DIRECTOR, NIH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 10/01/2001
PERIOD OF PERFORMANCE END DATE: 02/28/2026

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Research Resources for Model Amphibians - This application proposes to develop fundamental genomic and bioinformatic resources that will better enable studies of the axolotl (Ambystoma mexicanum). The axolotl provides the best model for identifying mechanisms of regeneration that can translate to humans. This is because it can regenerate multiple damaged body parts, including whole limbs, spinal cord, and brain, and because it shares tetrapod biology with human. Understanding how axolotls regenerate complex tissues may reveal mechanisms to activate stem cells within human tissues to organize and reform damaged tissues. Beyond regeneration, the axolotl presents many untapped disease models that were identified and rigorously examined with traditional developmental approaches before the genetics/genomics era. With our recent development of the first chromosome-scale assembly of the axolotl genome, these and new models that will be created in coming years through genome editing, can be advanced for the first time to significantly impact biomedical research. However, to advance these models, improvements of the genome assembly are needed along with new datasets to enable genome-wide studies of the large axolotl genome. To accomplish these objectives, we propose three specific aims. In Aim 1, we will resolve inter- and intra-specific variation within the current assembly and resolve complex repetitive regions that have hampered the placement of ~6 Gb of the 32 Gb genome. Specifically, we will employ a powerful trio binning strategy that takes advantage of long read sequencing of DNA from axolotl x tiger salamander (A. tigrinum) hybrids. This approach leverages decades of experience in generating interspecific hybrids to resolve assembly gaps/errors and accurately place gene models within their non-coding regulatory environments. Additionally, we will analyze sequence data from 10 AGSC axolotls from branches of the pedigree that optimally sample laboratory genetic diversity. In Aim 2, we will perform Chip-Seq to identify changes in histone post-translational modifications during tail and limb regeneration. Characterization of epigenetic marks for embryo, larval, and adult models of regeneration will provide new resources for regeneration research and advance community efforts in performing genome wide studies. Aim 3 will address significant community needs, user-friendly access to large scale genomic datasets and improvement of genomic and bioinformatic capabilities of investigators in the axolotl community. Accomplishment of these aims will significantly advance axolotl as an animal model for biomedical research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $406,029 )
2025	2025	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	23	2/14/2025	NON-COMPETING CONTINUATION	$406,029
														Subtotal = $406,029

Issue Date FY: 2024 ( Subtotal = $507,536 )
2024	2024	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	001	22	5/17/2024	NON-COMPETING CONTINUATION	$101,507
2024	2024	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	22	2/9/2024	NON-COMPETING CONTINUATION	$406,029
														Subtotal = $507,536

Issue Date FY: 2023 ( Subtotal = $624,804 )
2023	2023	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	002	21	2/15/2023	NON-COMPETING CONTINUATION	$499,886
2023	2023	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	004	21	7/19/2023	SUPPLEMENT FOR EXPANSION	$0
2023	2023	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	003	21	6/26/2023	SUPPLEMENT FOR EXPANSION	$124,918
2023	2022	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	001	20	11/7/2022	COMPETING CONTINUATION	$155,748
2023	2019	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	19	11/7/2022	NON-COMPETING CONTINUATION	-$155,748
														Subtotal = $624,804

Issue Date FY: 2022 ( Subtotal = $499,886 )
2022	2022	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	002	20	5/5/2022	COMPETING CONTINUATION	$55,949
2022	2022	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	001	20	3/15/2022	COMPETING CONTINUATION	$443,937
2022	2022	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	003	20	8/3/2022	COMPETING CONTINUATION	$0
2022	2019	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	19	3/14/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $499,886

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2019	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	19	7/21/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $493,287 )
2019	2019	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	001	19	7/13/2019	NON-COMPETING CONTINUATION	$0
2019	2019	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	19	7/5/2019	NON-COMPETING CONTINUATION	$493,287
														Subtotal = $493,287

Issue Date FY: 2018 ( Subtotal = $493,287 )
2018	2018	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	18	6/18/2018	NON-COMPETING CONTINUATION	$493,287
														Subtotal = $493,287

Issue Date FY: 2017 ( Subtotal = $468,622 )
2017	2017	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	001	17	7/17/2017	NON-COMPETING CONTINUATION	$24,662
2017	2017	UNIVERSITY OF KENTUCKY	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Research Infrastructure Programs	000	17	6/20/2017	NON-COMPETING CONTINUATION	$443,960
														Subtotal = $468,622

Issue Date FY: 2016 ( Subtotal = $493,287 )
2016	2016	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Research Infrastructure Programs	000	16	6/3/2016	COMPETING CONTINUATION	$493,287
														Subtotal = $493,287

Issue Date FY: 2015 ( Subtotal = $633,615 )
2015	2015	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Research Infrastructure Programs	001	15	8/13/2015	SUPPLEMENT FOR EXPANSION	$150,000
2015	2015	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Research Infrastructure Programs	000	15	7/7/2015	NON-COMPETING CONTINUATION	$483,615
														Subtotal = $633,615

Issue Date FY: 2014 ( Subtotal = $323,296 )
2014	2014	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Research Infrastructure Programs	000	14	6/21/2014	NON-COMPETING CONTINUATION	$323,296
														Subtotal = $323,296

Issue Date FY: 2013 ( Subtotal = $314,075 )
2013	2013	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Research Infrastructure Programs	000	13	7/31/2013	NON-COMPETING CONTINUATION	$314,075
														Subtotal = $314,075

Issue Date FY: 2012 ( Subtotal = $317,970 )
2012	2012	UNIVERSITY OF KENTUCKY, RESEARCH FOUNDATION	105 KINKEAD HALL	LEXINGTON	KY	40506	FAYETTE	USA	Trans-NIH Research Support	000	12	7/23/2012	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$317,970
														Subtotal = $317,970

Grand Total All Awards = $5,575,694

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Research Resources for Model Amphibians

Award Number: R24OD010435

ORGANIZATION: OFFICE OF THE DIRECTOR, NIH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 10/01/2001

PERIOD OF PERFORMANCE END DATE: 02/28/2026

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