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Analysis of RNAs targeted to the long-distance projections of ventral hippocampus

Award Number: R21MH133252
ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 12/05/2023
PERIOD OF PERFORMANCE END DATE: 11/30/2025

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Analysis of RNAs targeted to the long-distance projections of ventral hippocampus - PROJECT SUMMARY In this R21 exploratory/developmental grant application, we propose to develop and validate methodologies for in vivo analysis of RNA targeting in specific circuits, as well as investigating the regulation of these RNAs during long-term memory storage (LTM). The significance of RNAs localized to dendrites for local translation is well known. However, current methodologies do not provide accurate identification of RNAs targeted to distinct subcellular compartments. For example, several studies utilizing molecular analysis of microdissected dendrites or neuronal processes have identified RNAs in the distally localized neurons of diverse organisms, including sea slugs and mice. A major caveat of this approach is that the microdissected regions contain dendrites/neuronal processes from several different neurons as well as segments of interneurons and localized non-neuronal cells. Thus, this approach yields RNAs from dendrites of multiple neurons and non- neuronal cells, preventing our ability to identify the neuron-specific, distally targeted RNAs necessary to generate a more profound understanding of RNA localization and LTM. Since, learning and memory involves changes that are circuit-specific, the lack of robust and reliable methodologies to identify circuit-specific, targeted RNAs acts as a critical barrier to our understanding of RNA targeting in vivo, as well as the role that these RNAs play in both in LTM and disorders of memory. To address this critical knowledge gap, we here propose a strategy that starts with the expression of a tagged kinesin that mediates the transport of RNAs in specific neurons followed by immunoprecipitation of kinesin complexes from distinct neuronal projections and identification and rigorous characterization of associated RNAs. We anticipate that our kinesin-based strategy will yield deep insights into in vivo RNA targeting and their modulation by LTM.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $241,500 )
2025	2025	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Mental Health Research Grants	001	2	9/23/2025	NON-COMPETING CONTINUATION	$36,225
2025	2025	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Mental Health Research Grants	000	2	11/15/2024	NON-COMPETING CONTINUATION	$205,275
														Subtotal = $241,500

Issue Date FY: 2024 ( Subtotal = $289,800 )
2024	2024	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Mental Health Research Grants	000	1	12/5/2023	NEW	$289,800
														Subtotal = $289,800

Grand Total All Awards = $531,300

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Analysis of RNAs targeted to the long-distance projections of ventral hippocampus

Award Number: R21MH133252

ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 12/05/2023

PERIOD OF PERFORMANCE END DATE: 11/30/2025

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