Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Measuring and describing nucleosome remodeler sequence preferences

Award Number: R21HG012258
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2022
PERIOD OF PERFORMANCE END DATE: 08/31/2024

Group Awards By:

View Award Description

Measuring and describing nucleosome remodeler sequence preferences - PROJECT SUMMARY/ABSTRACT Hughes - “Measuring and describing nucleosome remodeler sequence preferences” Determining how cells interpret regulatory sequence is a difficult but important problem that broadly impacts disciplines including human genetics, development, and evolution. Computational approaches in this area are usually focused on transcription factor (TF) binding sites. There is substantial evidence, however, that nucleosome remodelers, which work together with TFs to generate open chromatin at regulatory sites, also possess some level of sequence specificity. This specificity could involve direct sequence recognition, ability to move or evict nucleosomes over some sequences but not others, and/or longer-range mechanisms such as sequence dependence of packing nucleosome arrays against a barrier. Here, we propose to develop methods to identify and describe the sequence dependence of each of these mechanisms, which can be applied to any remodeling enzyme. To avoid the complex and confounding effects of other factors, the methods will employ biochemical assays with purified components. The resulting data will then be interrogated to identify sequence features that correlate with and predict nucleosome occupancy and movement in response to each type of remodeler, and models constructed that can detect and score these features within any given sequence. These models can be used analogously to and in conjunction with widely used transcription factor motif models. If successful, these methods could be applied to the many variants of remodeling complexes. The resulting models should be widely applicable in the study of gene regulation, analogous to transcription factor DNA binding motifs. Development and validation of this method could therefore have widespread impact in human genetic analysis and broad applicability in molecular genetic research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2022	THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO	27 KING'S COLLEGE CIR	TORONTO	ON			CAN	Human Genome Research	000	1	12/29/2024	NEW	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $267,263 )
2022	2022	GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO	27 KING'S COLLEGE CIR	TORONTO	ON			CAN	Human Genome Research	000	1	8/31/2022	NEW	$267,263
														Subtotal = $267,263

Grand Total All Awards = $267,263

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Measuring and describing nucleosome remodeler sequence preferences

Award Number: R21HG012258

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2022

PERIOD OF PERFORMANCE END DATE: 08/31/2024

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer