Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Comprehensive mapping of pre-replication complex protein binding sites in single human cells

Award Number: R21HG010403
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/06/2018
PERIOD OF PERFORMANCE END DATE: 08/31/2021

Group Awards By:

View Award Description

Comprehensive mapping of pre-replication complex protein binding sites in single human cells - Abstract A major gap in genome research is the lack of comprehensive maps of human replication origins, a fundamental chromosome functional element. The inability to identify the locations of origins is a principle barrier to understanding cellular replication control and a glaring deficiency in maps of human functional elements. Methods to map either the fleeting initiation event or the sites of pre-replication complex (pre-RC) protein binding suffer from low signal-to-noise ratios that we hypothesize is due to the stochastic assembly of pre-RCs at different sites in different cells, precluding their detection using ensemble methods. Our long term goal is to create reliable high confidence maps of human pre-RC proteins and determine their probabilities of binding at any given site. The overall objective of this application is to assess the feasibility of two novel methods to produce single cell or at least low cell number comprehensive pre-RC protein maps. Our approach is to adapt emerging methods that bypass the poor efficiency of immunoprecipitation to single cell or at least low cell number mapping of pre-RC proteins, that we hypothesize will permit detection of their binding sites. Future integration of such maps with other genomics data would permit a comprehensive view of how replication is coordinated with other chromosome functions in development and disease. This work is significant because its success would fill a major gap in functional genome mapping while developing generalizable methods with broad potential for single cell protein binding site mapping. This proposal is innovative in that we adapt emerging methods to test a novel hypothesis. It is high risk because our prediction for why pre- RCs have not been mappable is speculative and methods to map protein binding sites in single cells are emergent; it is high pay-off because the ability to map pre-RC assembly sites would be transformative for the fields of genomics and DNA replication.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$15,069 )
2022	2019	FLORIDA STATE UNIVERSITY	874 TRADITIONS WAY	TALLAHASSEE	FL	32306	LEON	USA	Human Genome Research	000	2	12/21/2021	NON-COMPETING CONTINUATION	-$15,069
														Subtotal = -$15,069

Issue Date FY: 2019 ( Subtotal = $224,939 )
2019	2019	FLORIDA STATE UNIVERSITY	874 TRADITIONS WAY	TALLAHASSEE	FL	32306	LEON	USA	Human Genome Research	000	2	7/22/2019	NON-COMPETING CONTINUATION	$224,939
														Subtotal = $224,939

Issue Date FY: 2018 ( Subtotal = $185,569 )
2018	2018	FLORIDA STATE UNIVERSITY	874 TRADITIONS WAY	TALLAHASSEE	FL	32306	LEON	USA	Human Genome Research	000	1	9/6/2018	NEW	$185,569
														Subtotal = $185,569

Grand Total All Awards = $395,439

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Comprehensive mapping of pre-replication complex protein binding sites in single human cells

Award Number: R21HG010403

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/06/2018

PERIOD OF PERFORMANCE END DATE: 08/31/2021

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer