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A Novel Physiologically Based Pharmacokinetic (PBPK) Modeling Approach to Facilitate Safer Medications During Lactation

Award Number: R21HD115866
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2025
PERIOD OF PERFORMANCE END DATE: 03/31/2027

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A Novel Physiologically Based Pharmacokinetic (PBPK) Modeling Approach to Facilitate Safer Medications During Lactation - Summary/Abstract Drug transfer into breast milk has consistently been a safety concern for medication use in breastfeeding women. Studies have shown that women use an average of three different medicines during lactation. Knowing how much of a drug is secreted into breast milk is critical for ensuring the safe use of medications for lactating mothers. Carrier-mediated transport plays a crucial role in drug transfer into human milk, particularly through efflux transporters that actively transport their substrates into breast milk. However, many challenges remain on obtaining accurate information of drug transfer into breast milk. Our research endeavors in this area have made significant progress, including the development of a new in vitro to in vivo extrapolation (IVIVE) approach to predict milk-to-plasma ratios (M/P), a human normal mammary epithelial cell-based permeability assay, and a mechanistic physiologically based pharmacokinetic (PBPK) modeling approach incorporating breast cancer resistance protein (BCRP) transporter to predict drug pharmacokinetics in the milk. These findings demonstrate that better prediction of milk-to-plasma (M/P) ratio needs a systemic approach that contains reliable in vitro assays to generate extrapolable drug parameters and a robust IVIVE approach like PBPK modeling that can integrate physiology and drug characteristic to run proactive predictions for general population and design precision dosage regimens for certain populations. Therefore, we hypothesize that a systemic and generalized IVIVE and PBPK modeling approach will highly improve prediction accuracy of drug transfer into breast milk. Since efflux drug transporters present on mammary epithelium have a significant role on drug transfer into breastmilk and there is a huge knowledge gap of their role, two P-glycoprotein (P-gp) drug substrates (clarithromycin and digoxin), four BCRP drug substrates (bupropion, cimetidine, ciprofloxacin and nitrofurantoin), and three passive diffusion drugs (atenolol, metoprolol and tramadol) will be used as probe drugs for this project. We will develop a novel lactating-like mammary epithelium barrier from human induced pluripotent stem cells (hiPSCs) to determine drug transfer in vitro and determine the fraction unbound of drugs in the plasma and breast milk. We will isolate human mammary epithelial cells (hMECs) from human breast milk and stimulate MECs from human reduction mammoplasty to be lactocyte-like cells. These cells will be used for running a stable heavy isotope-labeled full-length protein internal standard (IS)-based targeted proteomics assay to quantify the absolute drug transporter expression for IVIVC. Finally, a mechanistic lactation PBPK models will be developed by incorporating in vitro parameters, scaling factors, physiological and drug parameters. This research will have a significant impact to support safer medication use during lactation and develop precision therapeutics during lactation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $448,182 )
2025	2025	THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK	4400 VESTAL PKWY E	BINGHAMTON	NY	13902	BROOME	USA	Child Health and Human Development Extramural Research	000	1	8/27/2025	NEW	$448,182
														Subtotal = $448,182

Grand Total All Awards = $448,182

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A Novel Physiologically Based Pharmacokinetic (PBPK) Modeling Approach to Facilitate Safer Medications During Lactation

Award Number: R21HD115866

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2025

PERIOD OF PERFORMANCE END DATE: 03/31/2027

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