Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

SEMI-SIMOA: A Chip-scale Flow-based Single-Molecule Assay at the Point-of-Care using Semiconductor Technology

Award Number: R21EB036154
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/05/2024
PERIOD OF PERFORMANCE END DATE: 04/30/2027

Group Awards By:

View Award Description

SEMI-SIMOA: A Chip-scale Flow-based Single-Molecule Assay at the Point-of-Care using Semiconductor Technology - Project Summary Over the past two decades, single-molecule assays (SIMOA) have transformed the detection landscape for low- abundance protein biomarkers, such as cytokines and neurological markers, across a vast dynamic range. Sourced from a variety of samples, these assays provide exceptional capabilities that enhance our knowledge of diseases and fine-tune diagnostic methodologies. While various platforms have been developed and commercialized, they invariably involve intricate workflows and depend on highly specialized, bulky equipment. Such complexities confine the use of this technology to centralized labs, necessitating controlled environments and skilled operators. To meet these challenges, we introduce a compact SIMOA system that seamlessly integrates electronics and sub-µm sized microfluidics within a single integrated circuit (IC) chip, produced by semiconductor foundries. The creation of the microfluidics leverages a unique single-step wet-etching process, which facilitates the efficient integration of thousands of fluidic and electronic channels onto a compact, millimeter-sized CMOS chip. Drawing from the principles of flow-cytometry-based and nanopore-centric SIMOA platforms, our system electronically counts individual sandwiched immunocomplexes as they pass through the on-chip 500-nm pores. Notably, the IC itself stands as the main instrument, enabling results to be directly viewed on a personal device, eliminating the need for a reader. The project objective will be achieved by four specific aims: (1) Aim I will focus on implementing the CMOS- embedded microfluidics, the sensing on-chip pores, integrated on-chip electrodes, and the magnetic separation module. (2) Aim II will focus on developing resistive-pulse-sensing low-noise readout circuits and signal processing units for digitization and identification of the binding events. The integrated microfluidics/electronics system will be packaged, tested, and validated. (3) Aim III will focus on the functionalization of nanoparticles using target-specific antibodies. We will target three biomarkers, ɑ-synuclein, NfL, and GFAP, that are related to neurodegenerative diseases. (4) Aim IV will optimize the workflow and validate the assay by benchmarking with the established commercial SIMOAs. This project holds significant promise; its successful completion could transform future diagnostics for many diseases that require detecting low-abundant biomarkers, particularly benefiting settings with limited resources.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $177,214 )
2026	2026	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	119 CALIFORNIA HALL	BERKELEY	CA	94720	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	4/27/2026	NON-COMPETING CONTINUATION	$177,214
														Subtotal = $177,214

Issue Date FY: 2025 ( Subtotal = $149,102 )
2025	2025	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	4/1/2025	NON-COMPETING CONTINUATION	$149,102
														Subtotal = $149,102

Issue Date FY: 2024 ( Subtotal = $149,101 )
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	7/5/2024	NEW	$149,101
														Subtotal = $149,101

Grand Total All Awards = $475,417

Top

All Categories

About

Search

Reports

Data Submission

Award Information

SEMI-SIMOA: A Chip-scale Flow-based Single-Molecule Assay at the Point-of-Care using Semiconductor Technology

Award Number: R21EB036154

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/05/2024

PERIOD OF PERFORMANCE END DATE: 04/30/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer