Monday, November 17, 2025 11/17/2025

Tumor subregion and immune cell type-selective proteomic profiling

Award Number: R21CA297400
ORGANIZATION: NATIONAL CANCER INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 12/01/2024
PERIOD OF PERFORMANCE END DATE: 11/30/2026

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Tumor subregion and immune cell type-selective proteomic profiling - Project Abstract A tumor is complex ecosystem in which cancer cells interact with diverse immune cells, creating a cancer- supportive microenvironment. The locations of these immune cells within a tumor significantly affect their functions, which are associated with cancer progression, metastasis, and response to therapy. We propose to develop a novel spatial proteomics approach by integrating 3D microscopy techniques with bottom-up proteomic assays, which can offer deep proteome profiles of specific immune cells at various subregions of a tumor. In Aim 1, we will develop a workflow involving 3D photobleaching-mediated spatial fluorescence encoding of immune cells located in different subregions of a mouse tumor. In Aim 2, we will apply the spatial proteomics method to identify macrophage niches associated with therapeutic responses in the tumor microenvironment. If successful, this innovative approach will revolutionize our ability not only to understand the spatially associated functions of immune cells in tumors but also to discover new immune cell niches as biomarkers and targets for accurate diagnosis and effective therapy for cancers.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $212,367 )
2025	2025	UNIVERSITY OF ILLINOIS	809 S MARSHFIELD AVE M/C 551	CHICAGO	IL	60612	COOK	USA	Cancer Biology Research	001	1	2/12/2025	NEW	$0
2025	2025	UNIVERSITY OF ILLINOIS	809 S MARSHFIELD AVE M/C 551	CHICAGO	IL	60612	COOK	USA	Cancer Biology Research	000	1	11/27/2024	NEW	$212,367
														Subtotal = $212,367

Grand Total All Awards = $212,367

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Tumor subregion and immune cell type-selective proteomic profiling

Award Number: R21CA297400

ORGANIZATION: NATIONAL CANCER INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 12/01/2024

PERIOD OF PERFORMANCE END DATE: 11/30/2026

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