Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

DNA-Hyaluronic Acid Platform for Spatiotemporally Probing the Role of Adhesion Ligands on BMP-Induced Osteogenesis In Vitro

Award Number: R21AR074069
ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2019
PERIOD OF PERFORMANCE END DATE: 01/31/2022

Group Awards By:

View Award Description

DNA-Hyaluronic Acid Platform for Spatiotemporally Probing the Role of Adhesion Ligands on BMP-Induced Osteogenesis In Vitro - Project Summary Advances in tissue engineering have been limited, in part, due to challenges in controlling the biomolecule signals important for regeneration. In particular, tissue engineering approaches for bone repair have been inhibited by the supraphysiological doses of the growth factor bone morphogenetic protein-2 (BMP-2) required for adequate healing due to suboptimal delivery. In the native extracellular matrix (ECM), BMP-2 is tightly bound by proteins and glycosaminoglycans with its presentation regulated in space and time to enhance bioactivity. Furthermore, researchers have shown significant synergies with other signaling molecules, such as cell-matrix or cell-cell adhesion ligands. To mimic these interactions, a number of elegant approaches based on photopatterning and orthogonal chemistries have been developed for presenting biomolecules. However, these approaches rely on highly customized chemical reactions (which may be difficult to implement with multiple classes of biomolecules) and are generally restricted to 1-2 signals. They are also often based on photocleavage reactions for temporal control, which precludes their reversibility over multiple cycles. Given the complexity of the extracellular environment (e.g. the stem cell “niche”) in controlling the fate of cells, a general in vitro platform that can control three or more signals, as well as multiple types of biomolecules, in both space and time would be invaluable for teasing apart the factors that control behavior like cell proliferation, migration, differentiation, and new tissue formation. We propose to develop a biomaterial-based in vitro platform capable of independently, and reversibly, controlling the spatiotemporal presentation of the growth factor bone morphogenetic protein-2 (BMP-2) in combination with the RGDS peptide to mimic cell-matrix adhesion and the HAVDI peptide to mimic cell-cell adhesion. Thus, the specific aims of the work are: (1) Investigate the temporal effect of immobilized BMP-2 on osteogenesis and (2) Determine the spatiotemporal role of cell-cell and cell- matrix interactions during BMP-2-induced osteogenesis.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$46 )
2022	2020	ARIZONA STATE UNIVERSITY	660 S MILL AVE STE 312	TEMPE	AZ	85281	MARICOPA	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	2	5/2/2022	NON-COMPETING CONTINUATION	-$46
														Subtotal = -$46

Issue Date FY: 2020 ( Subtotal = $151,922 )
2020	2020	ARIZONA STATE UNIVERSITY	660 S MILL AVE STE 312	TEMPE	AZ	85281	MARICOPA	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	2	1/27/2020	NON-COMPETING CONTINUATION	$136,729
2020	2020	ARIZONA STATE UNIVERSITY	660 S MILL AVE STE 312	TEMPE	AZ	85281	MARICOPA	USA	Arthritis, Musculoskeletal and Skin Diseases Research	001	2	4/14/2020	NON-COMPETING CONTINUATION	$15,193
														Subtotal = $151,922

Issue Date FY: 2019 ( Subtotal = $195,816 )
2019	2019	ARIZONA STATE UNIVERSITY	660 S MILL AVE STE 312	TEMPE	AZ	85281	MARICOPA	USA	Arthritis, Musculoskeletal and Skin Diseases Research	000	1	3/25/2019	NEW	$195,816
														Subtotal = $195,816

Grand Total All Awards = $347,692

Top

All Categories

About

Search

Reports

Data Submission

Award Information

DNA-Hyaluronic Acid Platform for Spatiotemporally Probing the Role of Adhesion Ligands on BMP-Induced Osteogenesis In Vitro

Award Number: R21AR074069

ORGANIZATION: NATIONAL INSTITUTE OF ARTHRITIS & MUSCULOSKELETAL & SKIN DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2019

PERIOD OF PERFORMANCE END DATE: 01/31/2022

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer