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Role of CLCC1 in herpesvirus nuclear egress

Award Number: R21AI196358
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/23/2026
PERIOD OF PERFORMANCE END DATE: 01/31/2028

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Role of CLCC1 in herpesvirus nuclear egress - PROJECT SUMMARY/ABSTRACT Nuclear egress – an unusual nuclear export process that herpesviruses use to translocate their capsids from the nucleus into the cytoplasm – is an essential early step in viral morphogenesis. During nuclear egress, capsids first bud at the inner nuclear membrane, acquiring a temporary envelope, which then fuses with the outer nuclear membrane, releasing the capsids into the cytoplasm. The budding stage is mediated by virally encoded proteins. However, what mediates the fusion stage is less clear. Our long-term goal is to elucidate the mechanism of nuclear egress in high-level detail. Previously, using a whole-genome CRISPR/Cas9 screen in combination with a custom flow-cytometry-based nuclear egress assay, we identified a new host factor, an ER protein CLCC1, as important for herpes simplex virus 1 (HSV-1) nuclear egress. Loss of CLCC1 caused a defect in nuclear egress and a drop in viral titers due to PEV accumulation in the PNS, implicating a defect at the fusion stage. Stable expression of CLCC1 in trans rescued all these defects. The goal of the current application is to uncover the mechanism by which CLCC1 facilitates membrane fusion at the nuclear envelope. Aim 1 will characterize the structure and clarify the oligomeric state of CLCC1. Aim 2 will investigate membrane-remodeling ability of CLCC1 using in-vitro assays. Biochemical and structural studies will be complemented with mutagenesis and viral infection phenotypes. The proposal is innovative because it explores an unusual mechanism, tests an innovative hypothesis, and is based on exciting preliminary data. The proposal is significant because it investigates the mysterious cellular factor that facilitates fusion of the nuclear envelope in health and disease. Upon successful completion, the proposed work will generate a mechanistic model of CLCC1 function that will set stage for in-depth exploration of its role in membrane fusion at the nuclear envelope.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $243,600 )
2026	2026	TRUSTEES OF TUFTS COLLEGE	136 HARRISON AVE	BOSTON	MA	02111	SUFFOLK	USA	Allergy and Infectious Diseases Research	000	1	2/23/2026	NEW	$243,600
														Subtotal = $243,600

Grand Total All Awards = $243,600

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Role of CLCC1 in herpesvirus nuclear egress

Award Number: R21AI196358

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/23/2026

PERIOD OF PERFORMANCE END DATE: 01/31/2028

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