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Single-cell transcriptional atlas of Giardia encystation

Award Number: R21AI188716
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/24/2025
PERIOD OF PERFORMANCE END DATE: 01/31/2027

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Single-cell transcriptional atlas of Giardia encystation - Project Summary Differentiation into dormant encapsulated cell types, known as encystation, is one of the most common modes of eukaryotic differentiation. Importantly, these changes cause the parasites to release from the host intestine, thus this process could be targeted to clear infections and/or block transmission. Despite its importance, very little is understood about the regulation of Giardia encystation. Here we propose to use single-cell RNA sequencing (scRNAseq) to build an atlas of gene expression during the cell cycle (Aim1) and during differentiation into cysts (Aim2). cAMP signaling is necessary and sufficient to initiate the encystation but not induce the formation of fully formed cysts. Hence, additional signals are required for cyst formation, and we aim to address this important question using scRNAseq. Together these studies will deepen our understanding of the encystation program and reveal unappreciated pathways that work to control the balance between persistence of infection versus transmission of this major parasite and model for the regulation of encystation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $218,767 )
2025	2025	UNIVERSITY OF WASHINGTON	4333 BROOKLYN AVE NE	SEATTLE	WA	98195	KING	USA	Allergy and Infectious Diseases Research	000	1	2/24/2025	NEW	$218,767
														Subtotal = $218,767

Grand Total All Awards = $218,767

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Single-cell transcriptional atlas of Giardia encystation

Award Number: R21AI188716

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/24/2025

PERIOD OF PERFORMANCE END DATE: 01/31/2027

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