Thursday, December 18, 2025 12/18/2025

CRISPR-Cas9 base editing approaches to discover ubiquitination events promoting flavivirus infection

Award Number: R21AI187731
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 11/14/2024
PERIOD OF PERFORMANCE END DATE: 10/31/2026

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CRISPR-Cas9 base editing approaches to discover ubiquitination events promoting flavivirus infection - PROJECT SUMMARY Viruses extensively remodel the cells that they infect. Ubiquitination networks are frequent targets of viral manipulation, but compared to other modifications such as phosphorylation, our general understanding of functional ubiquitination events is limited. In this project, we will apply CRISPR-Cas9 base-editing approaches to assess ubiquitination site function in a systematic, unbiased manner in the context of flavivirus infection. We have previously quantified ubiquitination responses to West Nile virus (WNV) infection, identifying hundreds of modulated ubiquitination targets with unknown functions. In Specific Aim 1, we will systematically introduce mutations at these ubiquitinated residues and assess the impact on WNV infection in a pooled base-editing screening format. In Specific Aim 2, we will apply base editing to perturb ubiquitination enzyme active sites for enzymes that physically interact with flavivirus NS5 proteins. Successful completion of these aims will discover novel pathways that promote flavivirus infection and will provide CRISPR-Cas9 base-editing resources tailored towards research questions in virology and ubiqutination functions.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $211,250 )
2026	2026	ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI	1 GUSTAVE L LEVY PL	NEW YORK	NY	10029	NEW YORK	USA	Allergy and Infectious Diseases Research	000	2	12/1/2025	NON-COMPETING CONTINUATION	$211,250
														Subtotal = $211,250

Issue Date FY: 2025 ( Subtotal = $245,895 )
2025	2025	ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI	1 GUSTAVE L LEVY PL	NEW YORK	NY	10029	NEW YORK	USA	Allergy and Infectious Diseases Research	000	1	11/14/2024	NEW	$245,895
														Subtotal = $245,895

Grand Total All Awards = $457,145

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CRISPR-Cas9 base editing approaches to discover ubiquitination events promoting flavivirus infection

Award Number: R21AI187731

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 11/14/2024

PERIOD OF PERFORMANCE END DATE: 10/31/2026

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