Monday, May 6, 2024 5/6/2024

Genetic screen to define the regulation of beta-hemolysin toxin expression in Streptococcus agalactiae

Award Number: R21AI178652
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By Issue Date FY or Funding FY:

View Award Abstract

Group B Streptococcus (GBS) is a normal constituent of the intestinal and vaginal flora in 15–30% of healthy adults. The bacterium can, however, cause infections in elderly and immunocompromised patients, and pregnant women, and is the main cause of fatal invasive disease in newborns. GBS translocation through epithelial, endothelial, and placental barriers is facilitated by a pore-forming toxin ß-hemolysin, also known as the ß-hemolytic pigmented lipid or granadaene. To identify factors involved in the regulation of ß-hemolysin expression we developed new peptide-free culture media for screening of a transposon mutant library of GBS CJB111. The pilot screens identified nine novel genetic loci associated with positive and negative modulation of ß-hemolysin expression. Based on preliminary findings, we hypothesize that GBS possesses a regulatory mechanism that tailors ß-hemolysin expression to the environment. To unravel the mechanism, we will employ transposon mutant library screening, RNA-seq and metabolomics analyses, isolation, and de novo sequence determination of secreted peptide-based repressors. The results of this exploratory work have the potential to lead to the development of a novel inhibitor of the invasion state of GBS.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $229,500 )
2024	2024	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE	LEXINGTON	KY	40526	FAYETTE	USA	Allergy and Infectious Diseases Research	000	2	4/16/2024	NON-COMPETING CONTINUATION	$229,500
														Subtotal = $229,500

Issue Date FY: 2023 ( Subtotal = $191,250 )
2023	2023	UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION, THE	500 S LIMESTONE 109 KINKEAD HALL	LEXINGTON	KY	40526	FAYETTE	USA	Allergy and Infectious Diseases Research	000	1	5/19/2023	NEW	$191,250
														Subtotal = $191,250

Grand Total All Awards = $420,750

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Genetic screen to define the regulation of beta-hemolysin toxin expression in Streptococcus agalactiae

Award Number: R21AI178652

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer