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Approaches for genetic manipulation of Naegleria fowleri

Award Number: R21AI175463
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/09/2023
PERIOD OF PERFORMANCE END DATE: 12/31/2025

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Approaches for genetic manipulation of Naegleria fowleri - Project Summary The goal of this proposal is to develop molecular tools and approaches for the study of the pathogenic free-living amoeba Naegleria fowleri. These currently do not exist. This amoeba is the causative agent of primary amebic meningoencephalitis (PAM), an almost invariable lethal brain infection. The lack of useful drugs for the treatment of this infection emphasizes the need to develop better therapeutic agents for amoebae infections. The lack of understanding of gene function in the parasite remains a major hurdle in target-based drug development campaigns. Here, we propose to address this problem by developing approaches for gene silencing and ablation by RNAi and CRISP/Cas9-mediate gene editing and transgene expression. These approaches will serve as an important first step in understanding both gene function and essentiality, key to early-stage drug discovery efforts.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $228,750 )
2024	2024	CLEMSON UNIVERSITY	201 SIKES HALL	CLEMSON	SC	29634	PICKENS	USA	Allergy and Infectious Diseases Research	000	2	11/9/2023	NON-COMPETING CONTINUATION	$205,875
2024	2024	CLEMSON UNIVERSITY	201 SIKES HALL	CLEMSON	SC	29634	PICKENS	USA	Allergy and Infectious Diseases Research	001	2	5/17/2024	NON-COMPETING CONTINUATION	$22,875
														Subtotal = $228,750

Issue Date FY: 2023 ( Subtotal = $190,625 )
2023	2023	CLEMSON UNIVERSITY	201 SIKES HALL	CLEMSON	SC	29634	PICKENS	USA	Allergy and Infectious Diseases Research	000	1	1/9/2023	NEW	$190,625
														Subtotal = $190,625

Grand Total All Awards = $419,375

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Approaches for genetic manipulation of Naegleria fowleri

Award Number: R21AI175463

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/09/2023

PERIOD OF PERFORMANCE END DATE: 12/31/2025

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