Monday, June 30, 2025 6/30/2025

Sodium Influx Assay for Measurement of TRPM7 Channel Activity in Intact Cells

Award Number: R21AI144337
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/01/2020
PERIOD OF PERFORMANCE END DATE: 05/31/2024

Group Awards By:

View Award Description

Sodium Influx Assay for Measurement of TRPM7 Channel Activity in Intact Cells - T lymphocyte activation and proliferation is essential for the human adaptive immune response. It is well established that calcium and magnesium signaling pathways play a crucial role in this process and their impairments result in various forms of immunodeficiency. Several players involved in calcium and magnesium influx have been identified at the molecular level. One of them, TRPM7, a protein with dual ion channel and serine/threonine kinase function is permeable to both calcium and magnesium. TRPM7 is required for T-cell development and activation of macrophages. Until now TRPM7 channel function has been studied primarily with patch-clamp electrophysiology. Detailed understanding of TRPM7 involvement in immune cellular signaling has been hampered by the lack of specific drug modulators of this channel. In order to develop effective high throughput drug screening assays for this purpose, novel ways of detecting channel activity in intact cells are necessary. This proposal is a proof-of-concept study of an intact cell fluorescence-based Na+ influx assay for measuring TRPM7 channel activity and its adaptation to high throughput format. The assay takes advantage of the biophysical properties of TRPM7 channels, specifically, drastic changes in current-voltage relation upon changes in extracellular divalent cation (Ca2+ or Mg2+) concentrations. Channel activity will be evaluated using intracellular Na+ concentrations as a readout. TRPM7 conduction and gating mutants identified electrophysiologically, will be used for Na+ influx assay validation. The Na+ flux assay will allow us to compare cellular Na+ changes in response to overexpression and mutagenesis of TRPM7. At the conclusion of the proposed studies we will have developed new tools for high throughput cell-based assays of TRPM7 for use in drug discovery.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$69,308 )
2025	2021	WRIGHT STATE UNIVERSITY	3640 COLONEL GLENN HWY	DAYTON	OH	45435	GREENE	USA	Allergy and Infectious Diseases Research	000	2	1/21/2025	NON-COMPETING CONTINUATION	-$69,308
														Subtotal = -$69,308

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2021	WRIGHT STATE UNIVERSITY	3640 COLONEL GLENN HWY	DAYTON	OH	45435	GREENE	USA	Allergy and Infectious Diseases Research	000	2	4/24/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $187,500 )
2021	2021	WRIGHT STATE UNIVERSITY	3640 COLONEL GLENN HWY	DAYTON	OH	45435	GREENE	USA	Allergy and Infectious Diseases Research	000	2	5/14/2021	NON-COMPETING CONTINUATION	$187,500
														Subtotal = $187,500

Issue Date FY: 2020 ( Subtotal = $221,000 )
2020	2020	WRIGHT STATE UNIVERSITY	3640 COLONEL GLENN HWY	DAYTON	OH	45435	GREENE	USA	Allergy and Infectious Diseases Research	000	1	5/26/2020	NEW	$221,000
														Subtotal = $221,000

Grand Total All Awards = $339,192

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Sodium Influx Assay for Measurement of TRPM7 Channel Activity in Intact Cells

Award Number: R21AI144337

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/01/2020

PERIOD OF PERFORMANCE END DATE: 05/31/2024

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer