Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Super-Resolution Optical Mapping for DNA Analysis Using Triplex-Forming Oligonucleotides as Stochastic Molecular Probes

Award Number: R15HG009972
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2018
PERIOD OF PERFORMANCE END DATE: 04/30/2026

Group Awards By:

View Award Description

Super-Resolution Optical Mapping for DNA Analysis Using Triplex-Forming Oligonucleotides as Stochastic Molecular Probes - Abstract Genome optical mapping is a quality control method for a genome sequencing project. The PI proposes a high- impact and innovative collaborative research project to develop non-invasive stochastic molecular probes for super-resolution genome optical mapping. Super-resolution genome optical mapping can significantly enhance the accuracy of the scaffolding information and can resolve the scaffolds at locations where traditional optical mapping cannot, thus significantly increasing the quality of a DNA assembly project. The traditional methods have a theoretical limitation at ~300 nm (or ~800 bases) resolution while the super-resolution techniques have no theoretical limit. However, there are several technical challenges and the PI has identified one to be the invasive fluorescent labeling methods used in the current optical mapping methods. In order to obtain an optical map, fluorescence tags have to be introduced to the target DNA at specific locations. In the two traditional optical mapping platforms, the target DNA is either fully cut by a restriction enzyme or one-strand cut by a nick labeling system. As such, the DNA breaks at locations with high labeling density, and the fragments are physically lost making super-resolution imaging at these locations impossible. The PI proposes to develop non-invasive fluorescence labeling probes as a solution to this problem. The goals of the proposal are: (1) apply super-resolution light microscopy in genome optical mapping; (2) develop non-invasive fluorescent labeling probes that have tunable labeling densities; (3) achieve stochastic fluorescent ON-OFF of the fluorescent tags for the super-resolution imaging; (4) enhance the research at Ohio University, and motivate and educate the undergraduate, graduate, and high school students in research. This project has introduced biochemical and biomedical research experience to underrepresented minority and female students in the Appalachian area, who would otherwise lack such opportunities. The students will experience techniques and knowledge that span widely in cell culture, DNA extraction, purification, and modification, DNA immobilization, probe design, probe synthesis, DNA labeling, traditional fluorescent imaging, super-resolution optical imaging, genome optical mapping, next-generation sequencing (NGS), and a variety of data analysis and bioinformatics methods.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $442,312 )
2023	2023	OHIO UNIVERSITY	1 OHIO UNIVERSITY	ATHENS	OH	45701	ATHENS	USA	Human Genome Research	001	2	4/26/2023	COMPETING CONTINUATION	$448,094
2023	2018	OHIO UNIVERSITY	1 OHIO UNIVERSITY	ATHENS	OH	45701	ATHENS	USA	Human Genome Research	000	1	2/21/2023	NEW	-$5,782
														Subtotal = $442,312

Issue Date FY: 2018 ( Subtotal = $451,682 )
2018	2018	OHIO UNIVERSITY	1 OHIO UNIVERSITY	ATHENS	OH	45701	ATHENS	USA	Human Genome Research	000	1	8/24/2018	NEW	$451,682
														Subtotal = $451,682

Grand Total All Awards = $893,994

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Super-Resolution Optical Mapping for DNA Analysis Using Triplex-Forming Oligonucleotides as Stochastic Molecular Probes

Award Number: R15HG009972

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2018

PERIOD OF PERFORMANCE END DATE: 04/30/2026

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer