Tuesday, July 1, 2025 7/1/2025

DEVELOPMENTAL ANALYSIS OF IMPRINTING MARK MODIFICATIONS

Award Number: R15HD041444
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2002
PERIOD OF PERFORMANCE END DATE: 02/28/2009

Group Awards By:

View Award Description

DEVELOPMENTAL ANALYSIS OF IMPRINTING MARK MODIFICATIONS - DESCRIPTION (provided by applicant): Genomic imprinting is a mammalian-specific phenomenon whereby the expression of a subset of genes is dependent on parental origin of the allele. Monoallelic expression is generally correlated with differential methylation of the parental alleles. Differences in methylation can be inherited as a primary imprinting mark from the gametes or acquired secondarily as a post-fertilization event. The significance of establishing the appropriate methylation pattern and achieving imprinted expression is illustrated by the fact that in the absence of parent of origin-specific expression, developmental defects ensue. Human diseases such as Prader-Willi, Angelman and Beckwith-Wiedemann syndromes result from the inappropriate expression of imprinted genes. The objective of this research is to enhance the understanding of how imprinted expression is achieved via an analysis of methylation acquisition at imprinted loci. The first goal is to conduct a temporal analysis of the acquisition of methylation during male gametogenesis at the paternally-methylated Gtl2 and Rasgrf1 loci. Second, the role of DNA methyltransferase 3L in establishing methylation at imprinted loci during male gametogenesis will be examined. Finally, the post-fertilization establishment of methylation at the Gtl2 promoter and its relationship to monoallelic expression will be investigated. Bisulfite mutagenesis and DNA sequencing will be the primary methodology utilized to examine methylation in these analyses. This research will advance the field of imprinting by determining whether the establishment of imprinting marks is globally or independently controlled. Furthermore, it will test the hypothesis that DNA methyltransferase 3L is the enzyme responsible for methylation establishment in the male germline. In addition, this project will enhance undergraduate education by providing students the opportunity to conduct independent research in molecular genetics.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2007 ( Subtotal = $56,661 )
2007	2007	BRYN MAWR COLLEGE	MERION DRIVE	BRYN MAWR	PA	19010	DELAWARE	USA	Child Health and Human Development Extramural Research	001	2	7/25/2007	SUPPLEMENT FOR EXPANSION	$56,661
														Subtotal = $56,661

Issue Date FY: 2005 ( Subtotal = $213,860 )
2005	2005	BRYN MAWR COLLEGE	MERION DRIVE	BRYN MAWR	PA	19010	DELAWARE	USA	Child Health and Human Development Extramural Research	000	2	3/3/2005	COMPETING CONTINUATION	$213,860
														Subtotal = $213,860

Issue Date FY: 2002 ( Subtotal = $126,618 )
2002	2002	BRYN MAWR COLLEGE	MERION DRIVE	BRYN MAWR	PA	19010	DELAWARE	USA	Child Health and Human Development Extramural Research	000	1	2/18/2002	NEW	$126,618
														Subtotal = $126,618

Grand Total All Awards = $397,139

Top

All Categories

About

Search

Reports

Data Submission

Award Information

DEVELOPMENTAL ANALYSIS OF IMPRINTING MARK MODIFICATIONS

Award Number: R15HD041444

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2002

PERIOD OF PERFORMANCE END DATE: 02/28/2009

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer