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Systematic Functional Genomics Analysis of the Oral Pathogen Streptococcus Mutans

Award Number: R15DE034550
ORGANIZATION: NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/24/2024
PERIOD OF PERFORMANCE END DATE: 09/24/2027

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Systematic Functional Genomics Analysis of the Oral Pathogen Streptococcus Mutans - Project Summary In this project we will construct a powerful new tool to systematically identify infection-relevant genes in the dental caries pathogen Streptococcus mutans. Sequence-defined arrayed mutant libraries are available for several medical and model bacterial species. These tools have greatly accelerated the pace of genetic screens and the identification of important pathogenic genetic determinants. We will construct an arrayed transposon mutant library in the model laboratory strain UA159 and use a sequencing approach to determine the identity of each mutant. After constructing the library, we will use it to study genetic determinants of sucrose-mediated biofilm formation, which is a critical virulence trait of S. mutans. We will also use a comparative transposon sequencing (Tn-seq) approach to identify genes that provide a selective advantage in biofilms. The results of this research will include the following: 1) construction of an arrayed and sequence defined transposon mutant resource for the S. mutans research community, 2) an improved understanding of the genetic determinants of sucrose-mediated biofilm formation both in monoculture and in complex communities, and 3) we expect this knowledge to contribute to the development of new approaches to target and reduce S. mutans colonization of the oral cavity. This project will also provide training opportunities for undergraduate and graduate students from Arkansas, an area that is underrepresented in biomedical research.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	ARKANSAS STATE UNIVERSITY	2105 AGGIE ROAD	JONESBORO	AR	72401	CRAIGHEAD	USA	Oral Diseases and Disorders Research	000	1	3/4/2025	NEW	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $419,161 )
2024	2024	ARKANSAS STATE UNIVERSITY	2105 AGGIE ROAD	JONESBORO	AR	72401	CRAIGHEAD	USA	Oral Diseases and Disorders Research	000	1	9/24/2024	NEW	$419,161
														Subtotal = $419,161

Grand Total All Awards = $419,161

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Systematic Functional Genomics Analysis of the Oral Pathogen Streptococcus Mutans

Award Number: R15DE034550

ORGANIZATION: NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/24/2024

PERIOD OF PERFORMANCE END DATE: 09/24/2027

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