Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

The role of H3K27me2 in regulating Culex pipiens diapause

Award Number: R15AI139861
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2018
PERIOD OF PERFORMANCE END DATE: 10/31/2025

Group Awards By:

View Award Description

The role of H3K27me2 in regulating Culex pipiens diapause - PROJECT SUMMARY Insects go through various evolutionary processes to survive in extreme environments and climates. Diapause is one of the most effective means that many insects adapted for a temperate environment. Our objective is to determine whether H3K27me2 regulates diapause traits including extended lifespan, fat accumulation, and enhanced stress resistance in the northern house mosquito, Cx. pipiens. We have three specific goals for this project: 1) To establish the link between diapause-relevant genes and H3K27me2 loss in fat body tissues. We will identify the genetic targets of the H3K27me2 and evaluate the genes’ putative roles in diapause traits using three functional assays: ChIP- seq, RNA-seq, and RNA interference (RNAi). 2) To identify and characterize the genes that are activated by H3K27me2 loss to generate the overwintering fat storage and consumption. We will identify dozens of specific types of lipids in the fat body cells during the overwintering periods using SSNMR and FTIR methods. Second, we will use KEGG Mapper to predict higher-level biochemical and functional pathways that are differentially expressed in fat body tissues exposed to the diapause-inducing condition. 3) To define the roles of the histone demethylase (UTX) and the histone methyltransferases (E(z) and ESC) in regulating H3K27me2 in order to understand the mechanisms that extend the lifespan of diapausing mosquitoes. We will characterize the role of utx, e(z) and esc in modulating H3K27 methylation and lifespan of diapausing females of Cx. pipiens. This will be achieved by knocking down the expression of these genes or by injecting chemical inhibitors that target the encoded proteins. We will monitor the impact on the H3K27 methylation levels and on the survival rates of the treated Cx. pipiens. Histone methylation is potentially a powerful target for breaking or reversing diapause-associated physiological changes. Like other epigenetic modifications, histone methylation is reversible, and thus by altering the activity of the enzyme that regulates these modifications, it may be possible to develop a novel control strategy to delay or prevent the onset of overwintering diapause in these mosquitoes and thus cause significant population loss during the winter season.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $516,916 )
2026	2026	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	000	3	5/26/2026	COMPETING CONTINUATION	$516,916
2026	2023	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	001	2	6/18/2026	COMPETING CONTINUATION	$0
														Subtotal = $516,916

Issue Date FY: 2024 ( Subtotal = -$4,847 )
2024	2023	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	000	2	10/1/2023	COMPETING CONTINUATION	-$4,847
														Subtotal = -$4,847

Issue Date FY: 2023 ( Subtotal = $443,169 )
2023	2023	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	002	2	6/27/2023	COMPETING CONTINUATION	$10,914
2023	2023	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	000	2	11/14/2022	COMPETING CONTINUATION	$443,169
2023	2018	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	001	1	6/27/2023	NEW	-$10,914
														Subtotal = $443,169

Issue Date FY: 2018 ( Subtotal = $346,250 )
2018	2018	BAYLOR UNIVERSITY	700 S UNIVERSITY PARKS DR	WACO	TX	76706	MCLENNAN	USA	Allergy and Infectious Diseases Research	000	1	5/1/2018	NEW	$346,250
														Subtotal = $346,250

Grand Total All Awards = $1,301,488

Top

All Categories

About

Search

Reports

Data Submission

Award Information

The role of H3K27me2 in regulating Culex pipiens diapause

Award Number: R15AI139861

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2018

PERIOD OF PERFORMANCE END DATE: 10/31/2025

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer