Friday, December 19, 2025 12/19/2025

Generation of an ILC3-specific Cre driver in mice

Award Number: R03AI182416
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/01/2024
PERIOD OF PERFORMANCE END DATE: 12/31/2025

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Generation of an ILC3-specific Cre driver in mice - SUMMARY This proposal aims to generate a Cre driver in mice that can be used to investigate the distinct functions and contributions of type 3 innate lymphoid cells (ILC3s) in immune responses and diseases. ILC3s, similar to CD4+ helper T lymphocytes (Th), play crucial roles in host defense and in maintaining tissue homeostasis. However, the functional overlap between ILC3s and Th17 cells has made it challenging to study their specific contributions in these and other contexts. Our research team identified an ILC3-specific enhancer (E22-2) associated with the Il22 gene, which encodes a type 3 signature cytokine. We now propose to generate a mouse strain with ILC3- specific expression of GFP-IRES-Cre using CRISPR-mediated targeting of a single-copy BAC-Il22 reporter (22- GFP) containing E22-2 and exhibiting GFP expression that is exquisitely restricted to mature ILC3s. The specificity and extent of Cre-mediated deletion will be determined by crossing the ILC3-specific Cre mice (22- GFPCre) with a flox-STOP-TdTomato reporter strain. Successful completion of this project will facilitate genetic engineering and targeted manipulation to study the ILC3-specific functions of specific factors without confounding effects on Th17 or iNKT17 cells. Examples of such future studies include establishing the roles of type 3 cytokines (IL-22 and IL-17A/F) in controlling infections, inflammatory bowel diseases, oncogenesis, and innate immune responses to cancer. Additionally, deployment of the new Cre driver will shed light on factors that govern the functional plasticity of ILC3s in inflammatory settings.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Allergy and Infectious Diseases Research	000	2	10/7/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $76,388 )
2025	2025	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Allergy and Infectious Diseases Research	000	2	12/12/2024	NON-COMPETING CONTINUATION	$76,388
														Subtotal = $76,388

Issue Date FY: 2024 ( Subtotal = $78,750 )
2024	2024	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Allergy and Infectious Diseases Research	001	1	9/11/2024	NEW	$7,875
2024	2024	OHIO STATE UNIVERSITY, THE	1960 KENNY RD	COLUMBUS	OH	43210	FRANKLIN	USA	Allergy and Infectious Diseases Research	000	1	1/29/2024	NEW	$70,875
														Subtotal = $78,750

Grand Total All Awards = $155,138

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Generation of an ILC3-specific Cre driver in mice

Award Number: R03AI182416

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/01/2024

PERIOD OF PERFORMANCE END DATE: 12/31/2025

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