Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

In vivo essential genome of Salmonella

Award Number: R03AI164158
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/18/2021
PERIOD OF PERFORMANCE END DATE: 07/31/2024

Group Awards By:

View Award Description

In vivo essential genome of Salmonella - SUMMARY Defining genome-wide genetic requirements for fitness of a bacterial pathogen in the host is a fundamental goal of pathogenomics. Transposon sequencing (Tn-seq) is a power functional genomics tool for genome-wide identification of essential and conditionally essential genes in bacteria. When applied to a bacterial pathogen with an appropriate animal infection model, Tn-seq can accelerate the discovery of the genetic determinants of a bacterial pathogen for in vivo fitness. However, the full potential of this powerful approach to understand bacterial virulence determinants is often hampered by the bottlenecks associated with animal infection models. The bottlenecks cause stochastic loss of the mutants without contribution of their genotypes, which can lead to false positive discoveries. In this application, we will develop a novel strategy to overcome this limitation by employing a transposon system that generates a genome-saturating random mutant library from the bacterial cells multiplied from a small seeder population established in the target host tissue upon induction, circumventing the bottleneck problem. The Tn-seq profile obtained from the genome-saturating mutant population recovered from the host tissue would allow genome-wide identification of in vivo essential genes by the genes lacking insertions in the same manner by which in vitro essential genes are identified. This new approach will be fully developed and evaluated (Aim1) and applied for genome-wide identification of the gut colonization factors of Salmonella Typhimurium 14028 during infection in mice (Aim 2). Once established and validated, this approach can be easily extended to other bacterial pathogens to fully explore in vivo essential genomes.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$9,494 )
2025	2022	DIVISION OF AGRICULTURE OF THE UNIVERSITY OF ARKANSAS	1371 W ALTHEIMER DR	FAYETTEVILLE	AR	72704	WASHINGTON	USA	Allergy and Infectious Diseases Research	000	2	3/10/2025	NON-COMPETING CONTINUATION	-$9,494
														Subtotal = -$9,494

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2022	DIVISION OF AGRICULTURE OF THE UNIVERSITY OF ARKANSAS	2301 S UNIVERSITY AVE	LITTLE ROCK	AR	72204	PULASKI	USA	Allergy and Infectious Diseases Research	000	2	8/24/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $71,763 )
2022	2022	UNIVERSITY OF ARKANSAS SYSTEM	1371 W ALTHEIMER DR	FAYETTEVILLE	AR	72704	WASHINGTON	USA	Allergy and Infectious Diseases Research	000	2	9/2/2022	NON-COMPETING CONTINUATION	$71,763
														Subtotal = $71,763

Issue Date FY: 2021 ( Subtotal = $71,763 )
2021	2021	UNIVERSITY OF ARKANSAS SYSTEM	1371 W ALTHEIMER DR	FAYETTEVILLE	AR	72704	WASHINGTON	USA	Allergy and Infectious Diseases Research	000	1	8/18/2021	NEW	$71,763
														Subtotal = $71,763

Grand Total All Awards = $134,032

Top

All Categories

About

Search

Reports

Data Submission

Award Information

In vivo essential genome of Salmonella

Award Number: R03AI164158

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/18/2021

PERIOD OF PERFORMANCE END DATE: 07/31/2024

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer