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GENETIC REGULATION OF THE TWIN ARGININE TRANSLOCATION SYSTEM IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM

Award Number: R03AI144661
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/19/2019
PERIOD OF PERFORMANCE END DATE: 12/31/2021

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GENETIC REGULATION OF THE TWIN ARGININE TRANSLOCATION SYSTEM IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM - Project Summary Salmonella species cause a range of diseases, from self-limiting gastroenteritis to life-threatening systemic infections, in a variety of hosts. The CDC estimates that non-typhoid Salmonella species cause 1.2 million cases of foodborne illness and 450 deaths per year in the United States alone. Salmonella enterica serovar Typhimurium is a leading cause of gastroenteritis worldwide and is used as a model for human typhoid fever in mice. The twin arginine translocation system (Tat) is a protein secretion system that is conserved in bacteria, archaea, and plants. In gram-negative bacteria, it is required for the export of substrate proteins from the cytoplasm to the periplasm of the organism. Tat substrates typically must be properly folded prior to export to the periplasm. In Salmonella, there are about 30 proteins that are substrates of Tat, among these are hydrogenases and amidases. While several studies have demonstrated that Tat is required for virulence in Salmonella and other bacterial pathogens, no published work has shown regulation of the system and tatABC is thought to be constitutively expressed. We have demonstrated that increasing concentrations of bile induce expression of a tatABC-lacZ fusion. Addition of 9% bile salt to LB media induces tatABC-lacZ about three-fold. We also have found that deletions of tatABC are more sensitive than wild type to peptidoglycan targeting antibiotics, though the addition of ampicillin does not activate expression of tatABC-lacZ. These finding leads to several questions: What is the mechanism of bile activation of tat expression? What promoter elements are important for expression of tatABC?


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2020	MIDWESTERN UNIVERSITY	19555 N 59TH AVE	GLENDALE	AZ	85308	MARICOPA	USA	Allergy and Infectious Diseases Research	000	3	7/18/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2019	SOUTHEAST MISSOURI STATE UNIVERSITY	ONE UNIVERSITY PLAZA	CAPE GIRARDEAU	MO	63701	CAPE GIRARDEAU	USA	Allergy and Infectious Diseases Research	000	1	12/10/2020	NEW	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $75,000 )
2020	2020	MIDWESTERN UNIVERSITY	19555 N 59TH AVE	GLENDALE	AZ	85308	MARICOPA	USA	Allergy and Infectious Diseases Research	000	3	1/16/2020	NON-COMPETING CONTINUATION	$75,000
														Subtotal = $75,000

Issue Date FY: 2019 ( Subtotal = $75,000 )
2019	2019	SOUTHEAST MISSOURI STATE UNIVERSITY	ONE UNIVERSITY PLAZA	CAPE GIRARDEAU	MO	63701	CAPE GIRARDEAU	USA	Allergy and Infectious Diseases Research	000	1	1/4/2019	NEW	$58,325
2019	2019	MIDWESTERN UNIVERSITY	19555 N 59TH AVE	GLENDALE	AZ	85308	MARICOPA	USA	Allergy and Infectious Diseases Research	002	2	7/8/2019	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$75,000
2019	2019	SOUTHEAST MISSOURI STATE UNIVERSITY	ONE UNIVERSITY PLAZA	CAPE GIRARDEAU	MO	63701	CAPE GIRARDEAU	USA	Allergy and Infectious Diseases Research	001	1	7/8/2019	NEW	-$58,325
														Subtotal = $75,000

Grand Total All Awards = $150,000

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GENETIC REGULATION OF THE TWIN ARGININE TRANSLOCATION SYSTEM IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM

Award Number: R03AI144661

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/19/2019

PERIOD OF PERFORMANCE END DATE: 12/31/2021

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