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DEVELOPMENT OF A CONTINOUS INVITRO CULTURE SYSTEM FOR PLASMODIUM VIVAX

Award Number: R03AI079475
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/03/2008
PERIOD OF PERFORMANCE END DATE: 06/30/2010

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DEVELOPMENT OF A CONTINOUS INVITRO CULTURE SYSTEM FOR PLASMODIUM VIVAX - DESCRIPTION (provided by applicant): The primary goal of this research project is to develop a continuous in vitro culture system for a human malaria parasite, Plasmodium vivax, which currently cannot be maintained in vitro for an extended period of time. The resulting system will be used for culturing P. vivax parasites from human patients to conduct important biological studies of P. vivax, such as drug resistance, and development of novel antimalarial drugs and vaccines. This will help with prevention and treatment of the disease caused by the parasite in endemic areas. To accomplish this goal, we will develop a system to obtain human reticulocytes, which is essential for P. vivax growth, by directing differentiation of the hematopoetic stem cells (HSCs) isolated from human umbilical cord blood (UCB), by using recombinant cell factors and co-culture with mouse or human stromal cells. The resulting reticulocytes will be infected with Plasmodium parasites (P. falciparum and P. vivax) to test if they can support growth of the parasites. The method for cryo- preservation of the cultured reticulocytes will also be studied for distribution of the reticulocytes to research laboratories and field sites. PUBLIC HEALTH RELEVANCE: The primary goal of this research project is to develop a continuous in vitro culture system of human reticulocytes for propagating a human malaria parasite, Plasmodium vivax. Currently, this system is not available and its absence impedes the development of drugs and/or vaccines for malaria caused by this parasite. To accomplish this goal, human reticulocytes will be developed through the directed differentiation of hematopoetic stem cells (HSCs) isolated from human umbilical cord blood (UCB). The enriched human reticulocytes will be tested for infection with Plasmodium parasites. Various methods for cryo- preservation of the cultured reticulocytes will be evaluated to create a qualified, long-term supply of the new culture system.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2009 ( Subtotal = $67,080 )
2009	2009	AMERICAN TYPE CULTURE COLLECTION	10801 UNIVERSITY BOULEVARD	MANASSAS	VA	20110	MANASSAS CITY	USA	Allergy and Infectious Diseases Research	000	2	7/15/2009	NON-COMPETING CONTINUATION	$67,080
														Subtotal = $67,080

Issue Date FY: 2008 ( Subtotal = $66,729 )
2008	2008	AMERICAN TYPE CULTURE COLLECTION	10801 UNIVERSITY BOULEVARD	MANASSAS	VA	20110	MANASSAS CITY	USA	Allergy and Infectious Diseases Research	000	1	7/3/2008	NEW	$66,729
														Subtotal = $66,729

Grand Total All Awards = $133,809

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DEVELOPMENT OF A CONTINOUS INVITRO CULTURE SYSTEM FOR PLASMODIUM VIVAX

Award Number: R03AI079475

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/03/2008

PERIOD OF PERFORMANCE END DATE: 06/30/2010

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