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IMMUNOPROTEOMICS AND DETECTION OF VIRAL DISEASES

Award Number: R03AI053718
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/15/2003
PERIOD OF PERFORMANCE END DATE: 07/31/2005

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IMMUNOPROTEOMICS AND DETECTION OF VIRAL DISEASES - DESCRIPTION (provided by applicant): Rapid detection of infectious agents has become more relevant with concerns associated with detection of bioterrorist agents. Current technology for detection and quantification of clinically relevant molecules is based on monoclonal and polyclonal antibodies used for immunoassay or detection of nucleic acids using RT-PCR systems. These assays are relatively slow taking at least 3 hours to complete. A novel, rapid, immunoproteomics assay based on high affinity specific antibodies coupled with a mass spectral read out is proposed. In a series of preliminary studies we have demonstrated the ability to use a single antibody-coated antigen capture and transfer reagent (ACTR) to detect model antigens, with good sensitivity, using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF). This immunoproteomic assay can be complete in < 1 hour and follows a sequential process in which antibody is immobilized in step 1, antigen is captured in step 2 and bound antigen is analyzed by mass spectrometry in step 3. Immunoproteomic assays may potentially be faster and as sensitive as the current antibody assays and could provide evidence for bacterial or viral antigens or specific antibodies to these products in samples for an infected animal. This RO3 proposal is designed to optimize this platform technology. In the initial proof-of-concept studies, we will develop and evaluate immunoproteomic assays to measure INF gamma (Th1 cytokine) and specific anti-viral antibodies in the serum of ectromelia infected mice. Ectromelia is a surrogate of variola virus, a CDC Category A bioterrorist agent and thus, the successful immunoproteomic approach may be relevant for detection of bioterrorist agents.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2004 ( Subtotal = $58,256 )
2004	2004	JUNIATA COLLEGE		HUNTINGDON	PA	16652	HUNTINGDON	USA	Allergy and Infectious Diseases Research	000	2	7/19/2004	NON-COMPETING CONTINUATION	$58,256
														Subtotal = $58,256

Issue Date FY: 2003 ( Subtotal = $58,256 )
2003	2003	JUNIATA COLLEGE		HUNTINGDON	PA	16652	HUNTINGDON	USA	Allergy and Infectious Diseases Research	000	1	8/12/2003	NEW	$58,256
														Subtotal = $58,256

Grand Total All Awards = $116,512

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IMMUNOPROTEOMICS AND DETECTION OF VIRAL DISEASES

Award Number: R03AI053718

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/15/2003

PERIOD OF PERFORMANCE END DATE: 07/31/2005

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