Wednesday, December 31, 2025 12/31/2025

Scalable high-plex pipeline for whole-brain proteomics profiling

Award Number: R01MH134803
ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/01/2025
PERIOD OF PERFORMANCE END DATE: 07/31/2028

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Scalable high-plex pipeline for whole-brain proteomics profiling - Abstract Knowing the molecular signature of distinct brain cell types in the human brain is relevant for basic neuroscience research and understanding neurological disease. While past and ongoing large-scale scRNAseq and spatial transcriptomics efforts have provided a comprehensive mRNA expression atlas, there lacks a similar proteomics atlas due to the limited profiling throughput of current methods. Additionally, studies have shown expression discrepancy between the transcription and translation levels, especially during development. Therefore, mapping proteomes, not just transcriptomes, is crucial to providing a comprehensive understanding of the cell-type molecular signatures. Current methods to profile the proteome have disadvantages such as low multiplexity, slow acquisition speed, and high data storage costs. We propose improving upon these current methods by increasing multiplexity with a 100- plex antibody panel, increasing acquisition speed with the creation of a novel ultra-speed light-sheet microscope and data management system, and lowering storage costs by implementing high-fidelity lossy compression. Finally, we will implement this scalable strategy to generate 100-plex proteomic atlases of the whole mouse brain across 6 postnatal stages.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	REGENTS OF THE UNIVERSITY OF MICHIGAN	1109 GEDDES AVE STE 3300	ANN ARBOR	MI	48109	WASHTENAW	USA	Mental Health Research Grants	000	1	11/19/2025	NEW	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $1,988,947 )
2025	2025	REGENTS OF THE UNIVERSITY OF MICHIGAN	1109 GEDDES AVE STE 3300	ANN ARBOR	MI	48109	WASHTENAW	USA	Mental Health Research Grants	000	1	7/23/2025	NEW	$1,988,947
														Subtotal = $1,988,947

Grand Total All Awards = $1,988,947

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Scalable high-plex pipeline for whole-brain proteomics profiling

Award Number: R01MH134803

ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/01/2025

PERIOD OF PERFORMANCE END DATE: 07/31/2028

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