Monday, June 30, 2025 6/30/2025

Postsynaptic roles of ankyrins

Award Number: R01MH107182
ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2015
PERIOD OF PERFORMANCE END DATE: 03/31/2027

Group Awards By:

View Award Description

Postsynaptic roles of ankyrins - ABSTRACT Extensive evidence implicates glutamatergic synapses as key pathogenic sites in neuropsychiatric disorders (NPDs). Recently-emerging evidence has implicated ankyrin proteins, including Ankyrin-G (AnkG), encoded by the ANK3 gene, and Ankyrin-B (AnkB), encoded by ANK2, in the biology of central nervous system synapses. While conventionally AnkG and –B have been seen as playing roles mainly at the axon initial segment, their functions in central glutamatergic synapses have only begun to be investigated, including in studies initiated by our laboratory. During the previous funding period, we have made extensive progress in understanding the regulation and interactome of AnkG at synapses and in dendrites. Our preliminary findings broaden our understanding of the neurobiology of ankyrins, and implicate them in several novel process, including deubiquitination and endocannabinoid signaling. In this proposal we will use neuroproteomics, conditional knockout and reporter mice, super-resolution and in vivo two-photon microscopy, calcium imaging, bioinformatics, and behavioral analyses, to investigate the functions of AnkG's protein partners in dendrite and at glutamatergic synapses. We hypothesize that AnkG performs multiple novel functions including supramolecular scaffold complex maintenance and regulation of endocannabinoid signaling, through which it controls spiny synapse development, structure, function, as well as circuit level network activity and behavior, in a cell type and developmental period-specific manner. We will test this hypothesis in the following Aims: Aim 1. To investigate the impact of cell type-dependent and developmentally-timed knock out of AnkG on brain structure, function, and behavior across maturation. Aim 2. To investigate the impact of cell type-dependent and developmentally-timed knock out of AnkB on brain structure, function, and behavior across maturation. Aim 3. To determine the mechanisms and functional significance of the interaction of AnkG with DAGLĮ. The proposed studies are highly novel and impactful, given that the synaptic functions of ankyrins are only beginning to be investigated. This proposal will be the first to investigate the function of several recently-discovered AnkG-interacting proteins in unexpected biological processes, using multiple cutting edge methods, and will open new avenues for many future studies.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $543,848 )
2025	2025	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	9	3/21/2025	NON-COMPETING CONTINUATION	$543,848
														Subtotal = $543,848

Issue Date FY: 2024 ( Subtotal = $639,821 )
2024	2024	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	8	4/5/2024	NON-COMPETING CONTINUATION	$575,838
2024	2024	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	001	8	8/15/2024	NON-COMPETING CONTINUATION	$63,983
														Subtotal = $639,821

Issue Date FY: 2023 ( Subtotal = $639,821 )
2023	2023	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	7	4/12/2023	NON-COMPETING CONTINUATION	$639,821
														Subtotal = $639,821

Issue Date FY: 2022 ( Subtotal = $639,821 )
2022	2022	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	6	5/26/2022	COMPETING CONTINUATION	$639,821
														Subtotal = $639,821

Issue Date FY: 2020 ( Subtotal = -$2,838 )
2020	2019	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	5	9/2/2020	NON-COMPETING CONTINUATION	-$2,838
														Subtotal = -$2,838

Issue Date FY: 2019 ( Subtotal = $464,866 )
2019	2019	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	5	4/3/2019	NON-COMPETING CONTINUATION	$464,866
														Subtotal = $464,866

Issue Date FY: 2018 ( Subtotal = $464,866 )
2018	2018	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	4	1/25/2018	NON-COMPETING CONTINUATION	$418,379
2018	2018	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	001	4	6/1/2018	NON-COMPETING CONTINUATION	$46,487
														Subtotal = $464,866

Issue Date FY: 2017 ( Subtotal = $464,866 )
2017	2017	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	001	3	7/25/2017	NON-COMPETING CONTINUATION	$46,487
2017	2017	NORTHWESTERN UNIVERSITY	633 CLARK ST	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	3	2/10/2017	NON-COMPETING CONTINUATION	$418,379
														Subtotal = $464,866

Issue Date FY: 2016 ( Subtotal = $464,866 )
2016	2016	NORTHWESTERN UNIVERSITY	633 CLARK STREET	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	2	2/6/2016	NON-COMPETING CONTINUATION	$464,866
														Subtotal = $464,866

Issue Date FY: 2015 ( Subtotal = $516,518 )
2015	2015	NORTHWESTERN UNIVERSITY	633 CLARK STREET	EVANSTON	IL	60208	COOK	USA	Mental Health Research Grants	000	1	2/17/2015	NEW	$516,518
														Subtotal = $516,518

Grand Total All Awards = $4,836,455

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Postsynaptic roles of ankyrins

Award Number: R01MH107182

ORGANIZATION: NATIONAL INSTITUTE OF MENTAL HEALTH

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2015

PERIOD OF PERFORMANCE END DATE: 03/31/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer