Tuesday, November 4, 2025 11/4/2025

Polarized Protein Trafficking and Angiogenesis

Award Number: R01HL155921
ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/01/2022
PERIOD OF PERFORMANCE END DATE: 12/31/2026

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Polarized Protein Trafficking and Angiogenesis - SUMMARY Blood vessels carry oxygen and nutrients and are vital to organismic viability and continued homeostasis. Angiogenesis, or the formation of new blood vessels from pre-existing ones, is the predominant developmental process by which blood vessel network density is regulated. During angiogenic development, endothelial cells create a hollow cavity called a lumen, providing a continuous conduit for blood to reach distant tissues. The mechanisms underpinning the morphodynamic changes in endothelial architecture and signaling leading to vascular lumen formation, or tubulogenesis, are incompletely understood. In this proposal we will investigate a protein called synaptotagmin-like protein 2 (sytl2) that we believe is responsible for deﬁning the luminal surface by directing protein transport to the apical membrane during blood vessel development. Our preliminary data suggests that sytl2 deﬁnes the apical membrane and tethers Rab GTPase proteins for delivery of vesicular cargo, such as podocalyxin. In aim 1, we will characterize the role of sytl2a during vascular lumen formation in developing zebraﬁsh embryos using a combination of live-imaging and CRISPR-based mutant generation. In aim 2, we will comprehensively demonstrate that sylt2 works in combination with the GTPase Rab35 to deliver podocalyxin to the apical plasma membrane during lumenogenesis in vitro. In aim 3, we will further characterized how sytl2a interacts with Rab35 to deliver Podocalyxin using generation of new zebraﬁsh reporter lines and compound mutants in vivo. How blood vessel lumen formation is regulated is still a major question in the ﬁeld, this proposal will provide novel insight into critical mechanisms orchestrating this process.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $360,584 )
2025	2025	UNIVERSITY OF DENVER	2199 S UNIVERSITY BLVD RM 222	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	000	4	12/16/2024	NON-COMPETING CONTINUATION	$360,584
														Subtotal = $360,584

Issue Date FY: 2024 ( Subtotal = $360,584 )
2024	2024	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	000	3	12/26/2023	NON-COMPETING CONTINUATION	$360,584
														Subtotal = $360,584

Issue Date FY: 2023 ( Subtotal = $360,584 )
2023	2023	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	000	2	1/6/2023	NON-COMPETING CONTINUATION	$324,526
2023	2023	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	001	2	3/6/2023	NON-COMPETING CONTINUATION	$36,058
														Subtotal = $360,584

Issue Date FY: 2022 ( Subtotal = $360,584 )
2022	2022	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	001	1	12/15/2021	NEW	$0
2022	2022	COLORADO SEMINARY	2199 S UNIVERSITY BLVD	DENVER	CO	80210	DENVER	USA	Cardiovascular Diseases Research	000	1	12/10/2021	NEW	$360,584
														Subtotal = $360,584

Grand Total All Awards = $1,442,336

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Polarized Protein Trafficking and Angiogenesis

Award Number: R01HL155921

ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/01/2022

PERIOD OF PERFORMANCE END DATE: 12/31/2026

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