Tuesday, July 1, 2025 7/1/2025

Light-Seq: Spatially targeted profiling of transcriptomic states in cells and tissue

Award Number: R01HG012926
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2023
PERIOD OF PERFORMANCE END DATE: 02/28/2027

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Light-Seq: Spatially targeted profiling of transcriptomic states in cells and tissue - Summary We will develop a new spatial-omics platform, Light-Seq, for spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Our light-directed barcoding strategy will enable user-directed, in situ selection of rare, disjoint cell populations for full- transcriptome sequencing based on morphology, location, or protein expression without dissociation. We will develop Light-Seq as a spatial-omic DNA barcoding platform capable of extracting the transcriptomic information from single-cells, scalable to uniquely address thousands of user-defined regions, and can be applied in both fixed and FFPE clinical samples for direct applications in human health. We envision that the Light-Seq platform will be a scalable, cost-effective, and flexible approach to spatial transcriptomics that allows the user to define spatial regions in tissue for NGS sequencing. Light-seq can thus serve as a low barrier-to-entry platform for spatial transcriptomics for many pathologists and researchers, and would be a key driver for a wider adoption of spatial transcriptomic tools.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $521,296 )
2025	2025	PRESIDENT AND FELLOWS OF HARVARD COLLEGE	1033 MASSACHUSETTS AVE STE 3	CAMBRIDGE	MA	02138	MIDDLESEX	USA	Human Genome Research	000	3	3/4/2025	NON-COMPETING CONTINUATION	$521,296
														Subtotal = $521,296

Issue Date FY: 2024 ( Subtotal = $580,022 )
2024	2024	PRESIDENT AND FELLOWS OF HARVARD COLLEGE	1033 MASSACHUSETTS AVE STE 3	CAMBRIDGE	MA	02138	MIDDLESEX	USA	Human Genome Research	001	2	6/13/2024	NON-COMPETING CONTINUATION	$47,348
2024	2024	PRESIDENT AND FELLOWS OF HARVARD COLLEGE	1033 MASSACHUSETTS AVE STE 3	CAMBRIDGE	MA	02138	MIDDLESEX	USA	Human Genome Research	000	2	2/27/2024	NON-COMPETING CONTINUATION	$532,674
														Subtotal = $580,022

Issue Date FY: 2023 ( Subtotal = $610,155 )
2023	2023	PRESIDENT AND FELLOWS OF HARVARD COLLEGE	1033 MASSACHUSETTS AVE 5TH FL	CAMBRIDGE	MA	02138	MIDDLESEX	USA	Human Genome Research	000	1	4/24/2023	NEW	$610,155
														Subtotal = $610,155

Grand Total All Awards = $1,711,473

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Light-Seq: Spatially targeted profiling of transcriptomic states in cells and tissue

Award Number: R01HG012926

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2023

PERIOD OF PERFORMANCE END DATE: 02/28/2027

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