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Development of methods for multi-omic analysis of DNA methylation and chromatin architecture in single cells

Award Number: R01HG010634
ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary/Abstract Chromatin organization and epigenomic modifications such as cytosine methylation play instrumental roles in gene regulation during human development and in healthy and disease states. Although the genome is nearly identical in each cell in the human body, chromatin conformation and cytosine DNA methylation are highly dynamic across cell types and during development. Previous studies showed that chromatin looping can be regulated by cytosine methylation. However, our current state of knowledge of the interactions between chromatin organization and DNA methylation is built on analyzing cultured cells and bulk tissues. The interaction between cell-type specific chromatin looping and methylation in heterogeneous tissues remain largely unexplored. This project will develop single-cell multi-omic methods to jointly analyze chromatin conformation and cytosine methylation from the same cell. The methods will lead to high quality cell-type specific chromatin conformation maps of human tissue, and the analysis of the relationship between chromatin conformation and cytosine methylation. If successful, the proposed methods will greatly facilitate the study of gene regulation in complex human tissues and in heterogeneous diseases such as cancer.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2022	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Human Genome Research	000	4	11/1/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $1,064,037 )
2022	2022	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Human Genome Research	000	4	7/20/2022	NON-COMPETING CONTINUATION	$1,064,037
														Subtotal = $1,064,037

Issue Date FY: 2021 ( Subtotal = $1,064,037 )
2021	2021	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Human Genome Research	000	3	6/23/2021	NON-COMPETING CONTINUATION	$1,064,037
														Subtotal = $1,064,037

Issue Date FY: 2020 ( Subtotal = $1,064,037 )
2020	2020	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Human Genome Research	000	2	6/22/2020	NON-COMPETING CONTINUATION	$1,064,037
														Subtotal = $1,064,037

Issue Date FY: 2019 ( Subtotal = $1,064,037 )
2019	2019	SALK INSTITUTE FOR BIOLOGICAL STUDIES, SAN DIEGO, CALIFORNIA, THE	10010 N TORREY PINES RD	LA JOLLA	CA	92037	SAN DIEGO	USA	Human Genome Research	000	1	9/5/2019	NEW	$1,064,037
														Subtotal = $1,064,037

Grand Total All Awards = $4,256,148

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Development of methods for multi-omic analysis of DNA methylation and chromatin architecture in single cells

Award Number: R01HG010634

ORGANIZATION: NATIONAL HUMAN GENOME RESEARCH INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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