Friday, March 6, 2026 3/6/2026

Mitochondrial function regulates ROS-mediated patterning following injury

Award Number: R01HD112474
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/15/2024
PERIOD OF PERFORMANCE END DATE: 02/28/2029

Group Awards By:

View Award Description

Mitochondrial function regulates ROS-mediated patterning following injury - Abstract Mitochondria are critical for generating energy. Recently a new role for mitochondria in controlling metabolic signaling through generation of reactive oxygen species (ROS) has come to light. ROS has also recently come into the spotlight as being a critical signaling axis for limb regeneration, and while mitochondrial signaling has been well-established in cell homeostasis, the role of mitochondrial generated ROS and its downstream mechanisms in controlling regeneration is not clear. Using the established mouse digit tip amputation regeneration model, spatial transcriptomics, and unique transgenic mice, we uncovered a unique role for two mitochondrial regulatory proteins, BNIP3 and NIX, in regeneration. Our preliminary findings support that regeneration is dependent on the mitochondrial regulation protein BNIP3, and that this protein supports ROS- generated, directional morphogenetic signaling gradients from the wound epithelium. Further, our preliminary data support that a second mitochondrial regulatory protein, NIX, serves to support blastema maturation and differentiation, a structure critical to regenerating the bone and soft tissue. We hypothesize that BNIP3 and NIX function in a spatially distinct relationship where NIX supports differentiation in the blastema in response to adjacent signaling gradients generated by BNIP3 in the wound epithelium. This project would provide the first evidence of a BNIP3/NIX signaling access in injury, implicating mitochondrial function and mitophagy as spatial regulators of the regeneration process, and giving a broader context to a mitochondrial homeostasis mechanism that is salient to many fields.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $562,524 )
2026	2026	MAINEHEALTH	110 FREE ST	PORTLAND	ME	04101	CUMBERLAND	USA	Child Health and Human Development Extramural Research	000	3	2/26/2026	NON-COMPETING CONTINUATION	$562,524
														Subtotal = $562,524

Issue Date FY: 2025 ( Subtotal = $562,524 )
2025	2025	MAINEHEALTH	110 FREE ST	PORTLAND	ME	04101	CUMBERLAND	USA	Child Health and Human Development Extramural Research	001	2	4/11/2025	NON-COMPETING CONTINUATION	$56,253
2025	2025	MAINEHEALTH	110 FREE ST	PORTLAND	ME	04101	CUMBERLAND	USA	Child Health and Human Development Extramural Research	000	2	3/3/2025	NON-COMPETING CONTINUATION	$506,271
														Subtotal = $562,524

Issue Date FY: 2024 ( Subtotal = $601,686 )
2024	2024	MAINEHEALTH	110 FREE ST	PORTLAND	ME	04101	CUMBERLAND	USA	Child Health and Human Development Extramural Research	000	1	3/13/2024	NEW	$601,686
														Subtotal = $601,686

Grand Total All Awards = $1,726,734

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Mitochondrial function regulates ROS-mediated patterning following injury

Award Number: R01HD112474

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/15/2024

PERIOD OF PERFORMANCE END DATE: 02/28/2029

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer