Sunday, November 24, 2024 11/24/2024

Hormonal Regulation Sertoli Cell Maturation

Award Number: R01HD010808
ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary/Abstract: The cycle of the seminiferous epithelium is a characteristic of spermatogenesis in mammals. The cycle serves to distribute the developmental stages of spermatogenesis in constant periodic intervals along the seminiferous tubules. The function of the cycle is to assure a constant source of spermatozoa over the relatively extended developmental time period. We have previously shown in the mouse testis that the cycle is initiated by pulses of retinoic acid (RA) originally generated by the Sertoli cells at stages VIII and IX of the cycle. The pulse of RA forces the undifferentiated spermatogonia to irreversibly enter the differentiation pathway. At stages VIII and IX we have shown in the adult mouse that the Sertoli cells demonstrate an increased abundance of hundreds of transcripts that allow for multiple functions such as RA synthesis, junction formation, uptake of residual bodies, spermiation, and others. In the absence of germ cells there are no cyclic activities of Sertoli cells detectable along the tubules, thus, accentuating the requirement for interactions between Sertoli and germ cells for normal spermatogenesis. We propose to examine the influence of spermatogonia at stages VIII and IX on the transcriptome of Sertoli cells and their functions during the development of complete spermatogenesis. We propose that this influence on the transcriptome and on the generation of the RA pulse is partially or wholly a result of Sertoli-germ cell interactions through the Notch signaling pathway. We will test this hypothesis by examining the influence of germ cells with Notch ligand gene deletions on the transcriptome and RA biosynthesis in normally developing Sertoli cells and in Sertoli cells with Notch receptor deletions. We will utilize a new technology designated capped small RNAseq or nTIseq to obtain information on active promoters and transcriptional start sites in the presence and absence of an intact Notch signaling pathway. We will pay special attention to transcripts regulating RA biosynthesis such as RDH10. The results of this study will clarify the interactive roles of germ and somatic cells in spermatogenesis and possibly implicate the Notch pathway in the etiology of male infertility.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	45	10/10/2024	COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $431,440 )
2024	2024	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	45	7/23/2024	COMPETING CONTINUATION	$431,440
														Subtotal = $431,440

Issue Date FY: 2022 ( Subtotal = $311,125 )
2022	2022	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	44	5/2/2022	NON-COMPETING CONTINUATION	$311,125
														Subtotal = $311,125

Issue Date FY: 2021 ( Subtotal = $311,125 )
2021	2021	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	43	3/12/2021	NON-COMPETING CONTINUATION	$311,125
														Subtotal = $311,125

Issue Date FY: 2020 ( Subtotal = $317,475 )
2020	2020	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	42	4/8/2020	NON-COMPETING CONTINUATION	$317,475
														Subtotal = $317,475

Issue Date FY: 2019 ( Subtotal = $317,475 )
2019	2019	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	41	4/16/2019	NON-COMPETING CONTINUATION	$317,475
														Subtotal = $317,475

Issue Date FY: 2018 ( Subtotal = $317,475 )
2018	2018	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	40	6/19/2018	COMPETING CONTINUATION	$317,475
2018	2017	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	39	6/19/2018	NON-COMPETING CONTINUATION	$0
2018	2017	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	002	39	9/11/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $317,475

Issue Date FY: 2017 ( Subtotal = $302,000 )
2017	2017	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	39	1/12/2017	NON-COMPETING CONTINUATION	$271,800
2017	2017	WASHINGTON STATE UNIVERSITY	240 FRENCH ADMINISTRATION BLDG	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	39	6/14/2017	NON-COMPETING CONTINUATION	$30,200
														Subtotal = $302,000

Issue Date FY: 2016 ( Subtotal = $357,825 )
2016	2016	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	38	1/7/2016	EXTENSION WITH OR WITHOUT FUNDS	$325,295
2016	2016	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	38	1/27/2016	EXTENSION WITH OR WITHOUT FUNDS	$48,369
2016	2016	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	002	38	2/4/2016	EXTENSION WITH OR WITHOUT FUNDS	-$15,839
														Subtotal = $357,825

Issue Date FY: 2015 ( Subtotal = $353,890 )
2015	2015	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	37	11/19/2014	NON-COMPETING CONTINUATION	$271,800
2015	2015	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	002	37	1/16/2015	NON-COMPETING CONTINUATION	$22,650
2015	2015	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	37	12/10/2014	SUPPLEMENT FOR EXPANSION	$59,440
														Subtotal = $353,890

Issue Date FY: 2014 ( Subtotal = $293,544 )
2014	2014	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	36	12/3/2013	NON-COMPETING CONTINUATION	$271,800
2014	2014	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	36	2/27/2014	NON-COMPETING CONTINUATION	$21,744
														Subtotal = $293,544

Issue Date FY: 2013 ( Subtotal = $302,000 )
2013	2013	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	35	1/7/2013	COMPETING CONTINUATION	$302,000
2013	2012	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	34	1/3/2013	NON-COMPETING CONTINUATION	$0
														Subtotal = $302,000

Issue Date FY: 2012 ( Subtotal = $284,170 )
2012	2012	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	34	3/5/2012	NON-COMPETING CONTINUATION	$284,170
														Subtotal = $284,170

Issue Date FY: 2011 ( Subtotal = $284,170 )
2011	2011	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	001	33	6/2/2011	NON-COMPETING CONTINUATION	$17,761
2011	2011	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	33	2/9/2011	NON-COMPETING CONTINUATION	$266,409
														Subtotal = $284,170

Issue Date FY: 2010 ( Subtotal = $369,474 )
2010	2010	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Trans-NIH Recovery Act Research Support	001	32	7/7/2010	SUPPLEMENT FOR EXPANSION	$73,464
2010	2010	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Child Health and Human Development Extramural Research	000	32	3/9/2010	NON-COMPETING CONTINUATION	$296,010
														Subtotal = $369,474

Issue Date FY: 2009 ( Subtotal = $299,000 )
2009	2009	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Population Research	001	31	4/21/2009	NON-COMPETING CONTINUATION	$29,900
2009	2009	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Population Research	000	31	2/18/2009	NON-COMPETING CONTINUATION	$269,100
														Subtotal = $299,000

Issue Date FY: 2008 ( Subtotal = $299,000 )
2008	2008	WASHINGTON STATE UNIVERSITY	FRENCH ADMIN BLDG, ROOM 240K	PULLMAN	WA	99164	WHITMAN	USA	Population Research	000	30	3/17/2008	COMPETING CONTINUATION	$299,000
														Subtotal = $299,000

Grand Total All Awards = $5,151,188

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Hormonal Regulation Sertoli Cell Maturation

Award Number: R01HD010808

ORGANIZATION: NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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