Tuesday, April 30, 2024
4/30/2024
Project Abstract/Summary Age-related macular degeneration (AMD) is the leading cause of blindness among older adults in the developed world. While recent studies support that mitochondrial (mt) defects in the retinal pigment epithelium (RPE) contribute to the pathogenesis of AMD, the causes of the dysfunction remains to be elucidated. Of the genetic polymorphisms linked to AMD, one of the most prevalent occurs in the gene encoding complement factor H (CFH). CFH is a negative regulator of the alternative complement pathway and protects against inappropriate complement activation that can cause chronic inflammation. The rs1061170 single-nucleotide polymorphism (SNP) in CFH, substituting a histidine at position 402 (Y402H) in the protein, is found in ~50% of AMD patients. How this single amino acid change contributes to AMD pathogenesis is unclear. Therefore, the goal of our proposed studies is to determine why the presence of the high risk CFH variant increases the prevalence of AMD disease. These studies build on our published work showing increased mtDNA damage in the RPE of AMD donors with the CFH high-risk SNP and a significant decrease in RPE mt function in induced pluripotent stem cell (iPSC)-RPE derived from donors harboring the high-risk allele. To accomplish our goal, we will use our collection of primary RPE and iPSC-derived RPE cultured from individuals phenotyped for AMD disease severity and genotyped for CFH risk. We will also use gene editing to create reciprocal isogenic iPSC-RPE lines from parent iPSCs containing either the CFH low or high risk allele. These model systems will be used to investigate how the presence of the CFH high risk allele alters RPE mt function and the response of RPE to metabolic and oxidative stressors. Aim 1 will test the hypothesis that intracellular complement regulates multiple cellular pathways that affect mt homeostasis and investigate how the presence of high risk 402H CFH variant protein alters these processes. Aim 2 will use a global metabolome and proteome approach to test the hypothesis that there are differences in metabolic and stress responsive pathways in RPE derived from donors with either the high or low risk CFH genotype. Findings from these studies will provide a comprehensive picture of the role of CFH in regulating RPE mt function by identifying cellular changes responsible for the loss in mt function and differences in stress response observed in RPE harboring the CFH high risk allele. This mechanistic insight could help explain the difference in AMD prevalence in CFH low versus high risk individuals. Knowledge about these differences may lead to development of therapies targeting the primary defect in a genetically defined population of AMD patients, which in turn, could lead to a “personalized medicine” approach for treatment of AMD.
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