Friday, March 14, 2025 3/14/2025

Mechanisms of photoreceptor protein transport and compartmentalization

Award Number: R01EY018421
ORGANIZATION: NATIONAL EYE INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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PROJECT SUMMARY/ABSTRACT The objectives of this proposal are to determine the mechanisms of photoreceptor protein compartmentalization. Retinal photoreceptors are polarized neurons whose major functions, include receiving and transmitting signals, are compartmentalized into discrete subcellular domains. Compartmentalization is critical for normal photoreceptor activity and reduced vision or blindness result from improper segregation of proteins. Despite their importance, the mechanisms underlying protein compartmentalization in photoreceptors, or any other neuron, remain poorly understood. Essential for understanding compartmentalization are the biophysical properties of the photoreceptor cytoplasm, the biophysical properties of the proteins that are destined to be compartmentalized and the forces that drive accumulation of proteins, against significant concentration gradients, into the specific compartments. We have uncovered a fundamental biophysical mechanism that may be central to protein transport and segregation in all electrically active cells: transport of charged proteins within the electrical field generated by the photoreceptor neuronal activity. We call this novel mechanism axial dynamic electrophoretic protein transport (ADEPT). We will use state of the art live cell fluorescence imaging tools developed in our lab, powerful transgenic and gene editing techniques in Xenopus, and sophisticated biochemical and cell biological approaches to address the following aims: Aim 1: Map the axial cytoplasmic electric field, Eax, in rod photoreceptors. Aim 2: Determine the impact of ADEPT on photoreceptor protein transport and compartmentalization in living photoreceptors. Aim 3: Determine the locations and influence of Arrestin interactions on their distributions and dynamics in living rods and cones.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $505,911 )
2025	2025	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	14	12/4/2024	NON-COMPETING CONTINUATION	$505,911
														Subtotal = $505,911

Issue Date FY: 2024 ( Subtotal = $505,911 )
2024	2024	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	13	11/29/2023	NON-COMPETING CONTINUATION	$505,911
														Subtotal = $505,911

Issue Date FY: 2023 ( Subtotal = $505,911 )
2023	2023	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	12	3/6/2023	NON-COMPETING CONTINUATION	$50,591
2023	2023	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	12	12/8/2022	NON-COMPETING CONTINUATION	$455,320
														Subtotal = $505,911

Issue Date FY: 2022 ( Subtotal = $491,348 )
2022	2022	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	11	12/8/2021	NON-COMPETING CONTINUATION	$455,888
2022	2022	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	11	5/4/2022	NON-COMPETING CONTINUATION	$35,460
														Subtotal = $491,348

Issue Date FY: 2021 ( Subtotal = $669,795 )
2021	2021	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	10	9/7/2021	SUPPLEMENT FOR EXPANSION	$63,985
2021	2021	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	10	12/10/2020	COMPETING CONTINUATION	$605,810
														Subtotal = $669,795

Issue Date FY: 2020 ( Subtotal = -$234 )
2020	2016	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	002	9	3/26/2020	EXTENSION WITH OR WITHOUT FUNDS	-$234
2020	2016	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	9	3/25/2020	EXTENSION WITH OR WITHOUT FUNDS	$234
2020	2015	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	8	3/25/2020	NON-COMPETING CONTINUATION	-$234
														Subtotal = -$234

Issue Date FY: 2019 ( Subtotal = -$215 )
2019	2016	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	9	2/6/2019	EXTENSION WITH OR WITHOUT FUNDS	-$215
														Subtotal = -$215

Issue Date FY: 2018 ( Subtotal = $0 )
2018	2016	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	9	2/26/2018	EXTENSION WITH OR WITHOUT FUNDS	$192
2018	2015	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	8	2/26/2018	NON-COMPETING CONTINUATION	-$192
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $0 )
2017	2016	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	001	9	3/2/2017	EXTENSION WITH OR WITHOUT FUNDS	$1,030
2017	2015	RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE	750 E ADAMS ST	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	8	3/2/2017	NON-COMPETING CONTINUATION	-$1,030
														Subtotal = $0

Issue Date FY: 2016 ( Subtotal = $398,750 )
2016	2016	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	P.O. BOX 9	ALBANY	NY	12201	ALBANY	USA	Vision Research	000	9	8/10/2016	EXTENSION WITH OR WITHOUT FUNDS	$398,750
														Subtotal = $398,750

Issue Date FY: 2015 ( Subtotal = $390,777 )
2015	2015	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	P.O. BOX 9	ALBANY	NY	12201	ALBANY	USA	Vision Research	000	8	8/31/2015	NON-COMPETING CONTINUATION	$390,777
														Subtotal = $390,777

Issue Date FY: 2014 ( Subtotal = $390,775 )
2014	2014	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	P.O. BOX 9	ALBANY	NY	12201	ALBANY	USA	Vision Research	000	7	8/26/2014	NON-COMPETING CONTINUATION	$390,775
														Subtotal = $390,775

Issue Date FY: 2013 ( Subtotal = $556,750 )
2013	2013	RESEARCH FOUNDATION OF STATE UNIV OF NEW YORK (SUNY)	P.O. BOX 9	ALBANY	NY	12201	ALBANY	USA	Vision Research	000	6	8/29/2013	COMPETING CONTINUATION	$556,750
														Subtotal = $556,750

Issue Date FY: 2011 ( Subtotal = $339,120 )
2011	2011	UNIVERSITY HOSPITAL OF UPSTATE MEDICAL CENTER	750 EAST ADAMS STREET	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	5	8/8/2011	NON-COMPETING CONTINUATION	$339,120
														Subtotal = $339,120

Issue Date FY: 2010 ( Subtotal = $349,718 )
2010	2010	UNIVERSITY HOSPITAL OF UPSTATE MEDICAL CENTER	750 EAST ADAMS STREET	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	4	8/5/2010	NON-COMPETING CONTINUATION	$349,718
														Subtotal = $349,718

Issue Date FY: 2009 ( Subtotal = $353,250 )
2009	2009	UNIVERSITY HOSPITAL OF UPSTATE MEDICAL CENTER	750 EAST ADAMS STREET	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	3	8/25/2009	NON-COMPETING CONTINUATION	$353,250
														Subtotal = $353,250

Issue Date FY: 2008 ( Subtotal = $346,185 )
2008	2008	UNIVERSITY HOSPITAL OF UPSTATE MEDICAL CENTER	750 EAST ADAMS STREET	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	2	9/3/2008	NON-COMPETING CONTINUATION	$346,185
														Subtotal = $346,185

Issue Date FY: 2007 ( Subtotal = $353,250 )
2007	2007	UNIVERSITY HOSPITAL OF UPSTATE MEDICAL CENTER	750 EAST ADAMS STREET	SYRACUSE	NY	13210	ONONDAGA	USA	Vision Research	000	1	8/20/2007	NEW	$353,250
														Subtotal = $353,250

Grand Total All Awards = $6,157,002

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Mechanisms of photoreceptor protein transport and compartmentalization

Award Number: R01EY018421

ORGANIZATION: NATIONAL EYE INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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