Thursday, July 17, 2025 7/17/2025

TOXIOLOGY OF PARTICULATE CR(VI)IN HUMAN LUNG CELLS

Award Number: R01ES010838
ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2001
PERIOD OF PERFORMANCE END DATE: 06/30/2006

Group Awards By:

View Award Description

TOXIOLOGY OF PARTICULATE CR(VI)IN HUMAN LUNG CELLS - Cr(VI) salts, particularly the insoluble compounds, are well-established human carcinogens, affecting the bronchial cells of the lung. No investigations have studied particulate Cr(VI) and only one has studied soluble Cr(VI) in human bronchial cells. Thus the goal of this research is to understand the mechanisms of Cr(VI)-induced carcinogenesis in human bronchial cells. The general hypothesis guiding this proposal is that particulate Cr(VI) is a more potent carcinogen than soluble Cr(VI) because it can escape cell cycle arrest at genotoxic doses. The specific goal of this project is to establish a human bronchial cell model to investigate these mechanisms. Three hypotheses will be tested: 1) Cr(VI) is genotoxic in human bronchial cells. 2) Particulate Cr(VI) compounds are more potent carcinogens than soluble Cr(VI) compounds because they provide chronic extracellular Cr(VI) exposure and escape cell cycle delay. 3) Particulate Cr(VI) salts escape cell cycle delay because of their divalent counter ion. These hypotheses will be tested with the following: 1) Cr(VI)-induced genotoxicity will be measured by the amount of damage produced in the Comet assay, and in metaphase chromosome spreads. Results will provide the first data on the carcinogenic and genotoxic effects of intact Cr(VI) particles to its target cells. 2) Particle uptake will be measured with transmission electron microscopy and ion uptake will be measured with inductively coupled plasma mass spectrometry. 3). Cell cycle effects will be measured with a mitotic index, fluorescent automated cell sorting and cDNA expression arrays. Results will the first reports of Cr(VI) toxicity in its target cells, the first detailed information of the interaction of Cr(VI) with the cell cycle, and will provide important toxicological data on the differences between particulate and soluble hexavalent chromium. This research is significant because it will provide: 1) an understanding of how Cr(VI) causes genetic in its target cells; 2) essential information to better assess the relative risk of exposure to particulate or soluble Cr(VI); 3) models of human bronchial cells for further study of Cr(VI), other metals, and lung cancer in general.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2005 ( Subtotal = $284,276 )
2005	2005	UNIVERSITY OF MAINE, PORTLAND-GORHAM	96 FALMOUTH STREET	PORTLAND	ME	04103	CUMBERLAND	USA	Applied Toxicological Research and Testing	000	5	6/15/2005	NON-COMPETING CONTINUATION	$284,276
														Subtotal = $284,276

Issue Date FY: 2004 ( Subtotal = $282,133 )
2004	2004	UNIVERSITY OF MAINE, PORTLAND-GORHAM	96 FALMOUTH STREET	PORTLAND	ME	04103	CUMBERLAND	USA	Applied Toxicological Research and Testing	000	4	6/18/2004	NON-COMPETING CONTINUATION	$282,133
														Subtotal = $282,133

Issue Date FY: 2003 ( Subtotal = $280,857 )
2003	2003	UNIVERSITY OF MAINE, PORTLAND-GORHAM	96 FALMOUTH STREET	PORTLAND	ME	04103	CUMBERLAND	USA	Applied Toxicological Research and Testing	000	3	6/13/2003	NON-COMPETING CONTINUATION	$280,857
														Subtotal = $280,857

Issue Date FY: 2002 ( Subtotal = $291,180 )
2002	2002	UNIVERSITY OF MAINE, PORTLAND-GORHAM	96 FALMOUTH STREET	PORTLAND	ME	04103	CUMBERLAND	USA	Applied Toxicological Research and Testing	000	2	9/10/2002	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$291,180
														Subtotal = $291,180

Issue Date FY: 2001 ( Subtotal = $291,440 )
2001	2001	YALE UNIVERSITY	PO BOX 208250	NEW HAVEN	CT	06520	SOUTH CENTRAL CT	USA	Applied Toxicological Research and Testing	000	1	8/22/2001	NEW	$291,440
														Subtotal = $291,440

Grand Total All Awards = $1,429,886

Top

All Categories

About

Search

Reports

Data Submission

Award Information

TOXIOLOGY OF PARTICULATE CR(VI)IN HUMAN LUNG CELLS

Award Number: R01ES010838

ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2001

PERIOD OF PERFORMANCE END DATE: 06/30/2006

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer