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Pyridoxal Radical Biocatalysis for the Stereoselective Production of Non-Canonical Amino Acids

Award Number: R01EB036084
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/10/2025
PERIOD OF PERFORMANCE END DATE: 03/31/2029

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Pyridoxal Radical Biocatalysis for the Stereoselective Production of Non-Canonical Amino Acids - Project Summary Biocatalysis represents a powerful emerging technology for the biomanufacturing of biologically active molecular agents and clinically significant therapeutics. Previously, the practice of biocatalysis is largely limited to the utilization and engineering of native enzymology from nature. New-to-nature enzymatic chemistries which are synthetically useful yet never encountered in biology remain largely underexploited. Combining biocatalysis and photochemistry, we propose a novel and generalizable photobiocatalysis strategy to reprogram diverse pyridoxal phosphate (PLP)-dependent enzymes as radical enzymes to catalyze stereoselective intermolecular radical reactions. Notably, these newly developed enzymatic reactions are not previously known in either biochemistry or organic chemistry. By merging photoredox catalysis powered by visible light and pyridoxal biocatalysis fine-tuned by directed evolution, a range of non-canonical amino acids that are often incorporated into medicines and therapeutic protein targets will be produced with excellent stereocontrol. By leveraging novel activation mechanisms involving open-shell PLP covalent intermediates, we will design and develop stereoselective biocatalytic radical C–C bond forming reactions at the a, b, or g position of abundant amino acid building blocks, furnishing an array of not easily accessible non-canonical amino acids, including those possessing a well-defined stereochemical dyad or triad which have remained out of the reach of traditional chemical and biological synthesis. Radical pyridoxal biocatalysis eliminates the need of tedious protecting group manipulation often required by traditional chemical synthesis, providing a modular, convergent and highly stereoselective approach to manufacturing biomedically valuable non-canonical amino acids.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $465,963 )
2026	2026	UNIVERSITY OF CALIFORNIA, SANTA BARBARA	3227 CHEADLE HALL	SANTA BARBARA	CA	93106	SANTA BARBARA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	3/25/2026	NON-COMPETING CONTINUATION	$465,963
														Subtotal = $465,963

Issue Date FY: 2025 ( Subtotal = $553,775 )
2025	2025	UNIVERSITY OF CALIFORNIA, SANTA BARBARA	3227 CHEADLE HALL	SANTA BARBARA	CA	93106	SANTA BARBARA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	4/9/2025	NEW	$553,775
														Subtotal = $553,775

Grand Total All Awards = $1,019,738

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Pyridoxal Radical Biocatalysis for the Stereoselective Production of Non-Canonical Amino Acids

Award Number: R01EB036084

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/10/2025

PERIOD OF PERFORMANCE END DATE: 03/31/2029

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