Wednesday, October 1, 2025 10/1/2025

Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue

Award Number: R01EB034921
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2024
PERIOD OF PERFORMANCE END DATE: 08/31/2027

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Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue - PROJECT SUMMARY We propose to develop and disseminate an advanced 2p Bessel beam light sheet (2pBLS) microscopy instrument for high-speed sub-micron-resolution volumetric imaging in deep tissue. The proposed instrument will have a significantly higher volume imaging speed over a large field of view, maintain a sub-micron diffraction- limited resolution in deep tissue despite the presence of tissue scatter and aberration, and perform simultaneous lifetime imaging with FRET sensors and high-speed light sheet imaging of dynamics intensity-modulated sensors for dual functional imaging in deep tissue. The system will be tested with two imaging studies in zebrafish and mouse respectively. In zebrafish, we will demonstrate live FLIM imaging of genetically encoded FRET sensors in zebrafish kidney and perform an imaging study of Ca2+ and cAMP activities in zebrafish renal cells. In mice, we will demonstrate imaging of neural and glial activities genetically labeled with activity sensors (Ca2+ and GEVI), FLIM-FRET sensors (PKA) and structural markers ex vivo and in vivo.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $849,527 )
2025	2025	UNIVERSITY OF ARIZONA	845 N PARK AVE RM 538	TUCSON	AZ	85721	PIMA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	2	8/19/2025	NON-COMPETING CONTINUATION	$522,738
2025	2024	UNIVERSITY OF ARIZONA	845 N PARK AVE RM 538	TUCSON	AZ	85721	PIMA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	3/28/2025	NEW	$326,789
														Subtotal = $849,527

Issue Date FY: 2024 ( Subtotal = $505,582 )
2024	2024	UNIVERSITY OF ARIZONA	845 N PARK AVE RM 538	TUCSON	AZ	85721	PIMA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	8/26/2024	NEW	$505,582
														Subtotal = $505,582

Grand Total All Awards = $1,355,109

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Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue

Award Number: R01EB034921

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2024

PERIOD OF PERFORMANCE END DATE: 08/31/2027

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