Sunday, January 25, 2026 1/25/2026

High-content High-speed Chemical Imaging of Metabolic Reprogramming by Integration of Advanced Instrumentation and Data Science

Award Number: R01EB032391
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2022
PERIOD OF PERFORMANCE END DATE: 12/31/2026

Group Awards By:

View Award Description

High-content High-speed Chemical Imaging of Metabolic Reprogramming by Integration of Advanced Instrumentation and Data Science - Project Summary: Providing molecular fingerprint vibration information and high imaging speed, coherent Raman scattering microscopy, based on either coherent anti-Stokes Raman scattering (CARS) or stimulated Raman scattering (SRS), allows real-time vibrational imaging of living cells and/or tissues with sub-micron spatial resolution These instrumentation-based advances, however, do not fulfill all the desired parameters in hyperspectral imaging, including broad bandwidth, high signal to noise ratio (SNR) and high speed. In pushing these physical limits, it is common that one parameter is optimized at the price of sacrificing other advantages. The current proposal aims to break this conventional thinking of no free lunch in optimization through a synergistic integration of advanced instrumentation and data science. A multidisciplinary team with a strong track record of collaborations will pursue the proposed studies. Ji-Xin Cheng (PI) is a leading expert in the development and applications of SRS chemical imaging. Lei Tian (co-I) is a leading expert in computational microscopy and machine learning. Daniela Matei (co-I) is a leading expert in cancer research specialized in ovarian cancer. We aim to develop two complementary platforms that will allow high-speed, high-content, and high-sensitivity mapping of cell metabolism. The first platform is for samples without prior knowledge. We will build a polygon scanner to tune the delay between two chirped pulses on a 20-microsecond time scale. We will then deploy deep spatial-spectral learning to denoise the low-SNR hyperspectral measurements and extract salient information with much enhanced SNR. This integrated approach effectively bypasses the conventional tradeoff between acquisition speed and SNR and enables high-speed, high-throughput, hyperspectral SRS imaging using informative fingerprint Raman bands. The second platform is for samples with known target species. We will develop a sparsely sampled hyperspectral imaging strategy to increase the overall speed by one order of magnitude while maintaining the same SNR. We will develop a novel recursive feature elimination approach to determine the minimum number of essential frames. On the instrumentation side, a fast-tuning fiber laser will be deployed to acquire a sparsely sampled hyperspectral stack within one second for the study of living systems. As a focused application, we will apply the proposed platforms to systematically investigate metabolic reprogramming in ovarian cancers that are cisplatin resistant. Our focused application will unveil hidden signatures that are associated with drug resistance, which will open new opportunities for improved treatment of drug-resistant cancers.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	11/13/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $504,866 )
2025	2025	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	12/20/2024	NON-COMPETING CONTINUATION	$454,378
2025	2025	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	4	5/19/2025	NON-COMPETING CONTINUATION	$50,488
														Subtotal = $504,866

Issue Date FY: 2024 ( Subtotal = $494,768 )
2024	2024	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	12/25/2023	NON-COMPETING CONTINUATION	$454,378
2024	2024	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	3	5/27/2024	NON-COMPETING CONTINUATION	$40,390
														Subtotal = $494,768

Issue Date FY: 2023 ( Subtotal = $456,157 )
2023	2023	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	2	1/31/2023	NON-COMPETING CONTINUATION	$45,617
2023	2023	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	12/22/2022	NON-COMPETING CONTINUATION	$410,540
														Subtotal = $456,157

Issue Date FY: 2022 ( Subtotal = $520,393 )
2022	2022	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	1	6/30/2022	NEW	$61,222
2022	2022	TRUSTEES OF BOSTON UNIVERSITY	1 SILBER WAY	BOSTON	MA	02215	SUFFOLK	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	12/21/2021	NEW	$459,171
														Subtotal = $520,393

Grand Total All Awards = $1,976,184

Top

All Categories

About

Search

Reports

Data Submission

Award Information

High-content High-speed Chemical Imaging of Metabolic Reprogramming by Integration of Advanced Instrumentation and Data Science

Award Number: R01EB032391

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2022

PERIOD OF PERFORMANCE END DATE: 12/31/2026

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer