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Mechanical Phenotyping of Random Periaerolar Fine Needle Aspiration-Collected Cells for Early Breast Cancer Detection

Award Number: R01EB024989
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary Early detection of breast cancer, especially those with high risk, would greatly improve outcomes. One method under current evaluation for early breast-cancer detection is random periareolar fine needle aspiration (RPFNA). RPFNA is based on the assumption that widespread cellular changes in the breast can be detected by random-tissue sampling. Cytopathology is performed on the collected epithelial cells to determine pre- cancerous changes; however, this analysis is only semi-quantitative. We propose to develop a point-of-care (POC), label-free platform to mechanically phenotype epithelial cells collected from RPFNAs to thus determine the presence of cancer cells and/or changes in cells that would indicate the likelihood of cancer. Our platform will be based on a novel microfluidic method we call “mechano-Node-Pore Sensing” (mechano-NPS). Mechano-NPS utilizes a node-pore sensor with a microfluidic contraction channel to measure simultaneously a single cell’s diameter, resistance to compressive deformation, transverse deformation, and recovery from deformation. We have used this multi- dimensional method of mechanical phenotyping to differentiate malignant vs. non-malignant epithelial cells, distinguish cells treated or untreated with cytoskeletal-perturbing small molecules, and discriminate between sub-lineages of normal primary human epithelial cells (HMECs). Importantly, we have used mechano-NPS to identify mechanical phenotypes that correlate with chronological age and malignant progression. Thus, we hypothesize that mechano-NPS and its ability to mechanically phenotype cells could potentially be used for early disease detection. We intend to demonstrate the full potential of our platform ability to distinguish normal cells from transformed ones by screening de-identified RPFNA patient samples. PI Lydia L. Sohn, Professor of Mechanical Engineering at UC Berkeley and Core Member of the UCSF-UC Berkeley Joint Graduate Group in Bioengineering will lead this NIH R01 project with PI, Mark LaBarge, who is a Professor of Population Science and an expert in breast-cancer biology at City of Hope. Sohn will lead the development of the platform with Key Personnel, Michael Lustig, Associate Professor of Electrical Engineering & Computer Sciences at UC Berkeley. LaBarge will provide guidance on sample choice, experimental design, data analysis, and clinical relevance.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $631,530 )
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	003	6	3/29/2024	SUPPLEMENT FOR EXPANSION	$83,565
2024	2024	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	002	6	3/1/2024	NON-COMPETING CONTINUATION	$547,965
2024	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	5	12/6/2023	SUPPLEMENT FOR EXPANSION	$0
2024	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	5	12/6/2023	COMPETING CONTINUATION	$0
														Subtotal = $631,530

Issue Date FY: 2023 ( Subtotal = $839,013 )
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	003	5	7/17/2023	COMPETING CONTINUATION	$0
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	5	2/23/2023	COMPETING CONTINUATION	$653,700
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	002	5	7/14/2023	SUPPLEMENT FOR EXPANSION	$137,312
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	004	5	9/1/2023	SUPPLEMENT FOR EXPANSION	$48,083
2023	2020	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 201	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	10/27/2022	NON-COMPETING CONTINUATION	-$82
														Subtotal = $839,013

Issue Date FY: 2020 ( Subtotal = $529,539 )
2020	2020	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 220	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	4/17/2020	NON-COMPETING CONTINUATION	$529,539
														Subtotal = $529,539

Issue Date FY: 2019 ( Subtotal = $529,539 )
2019	2019	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1608 4TH ST STE 220	BERKELEY	CA	94710	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	5/1/2019	NON-COMPETING CONTINUATION	$529,539
														Subtotal = $529,539

Issue Date FY: 2018 ( Subtotal = $513,398 )
2018	2018	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	2150 SHATTUCK AVE RM 313	BERKELEY	CA	94704	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	4/13/2018	NON-COMPETING CONTINUATION	$513,398
														Subtotal = $513,398

Issue Date FY: 2017 ( Subtotal = $527,648 )
2017	2017	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	2150 SHATTUCK AVE RM 313	BERKELEY	CA	94704	ALAMEDA	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	7/31/2017	NEW	$527,648
														Subtotal = $527,648

Grand Total All Awards = $3,570,667

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Mechanical Phenotyping of Random Periaerolar Fine Needle Aspiration-Collected Cells for Early Breast Cancer Detection

Award Number: R01EB024989

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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