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Minimally-Invasive Metabolite Monitoring Systems for in vitro and in vivo Disease Models

Award Number: R01EB024601
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary/Abstract Monitoring the state of a biological system in terms of biochemical composition is of significance to many research and patient care situations, from controlling bioreactors and in vitro cell culture systems to animal subjects and human patients. Metabolite monitoring has become essential to basic understanding of biological processes and the ability to track metabolic changes over time is considered critical to precision medicine. However, current approaches to such measurements are limited in availability, flexibility, and general functionality or are simply too costly or complex for use in many situations. A versatile, expandable approach to provide specific, frequent (real-time/on-demand), and stable measurements of various metabolic biomarkers is needed to facilitate high-throughput data collection on small-molecule metabolites, as this will lead to enhanced understanding of and control over a wide variety of biological systems. We propose to address this problem with a versatile metabolite sensing platform based on a pairing of biocompatible “smart” materials with customized optical instrumentation. Specifically, phosphorescent hydrogels and miniaturized optical readers will be developed. A novel materials approach will be applied to produce hydrogels with embedded spherical microdomains to precisely control diffusion-reaction processes, providing control over enzymatic operation at different substrate supply rates. The hydrogel provides a stable biocompatible interface to the surrounding environment (tissue/cells, etc.) and maintains the microdomains in a fixed location so they may not migrate and may be removed at a later point. The materials are moldable into form factors allowing attachment to culture substrates or needle insertion into organ cultures or living tissue. This new sensing platform requires successful integration of two core features that connect directly to the Specific Aims: 1) robust hydrogel-based phosphorescent sensors that can integrate directly into any biological system (tissue, cell culture, etc) and 2) robust optical measurement systems that can be miniaturized and directly interfaced with any relevant biological system. Stable, biocompatible hydrogels functionalized with oxygen- sensitive phosphors will be designed to integrate with in vitro and in vivo disease models (Specific Aim 1). Design rules for enzymatic hydrogels to function in different environments will be established, enabling materials that will respond in proportion to concentration of target analytes (Specific Aim 2). Reference materials will be incorporated to ensure accurate and robust analysis (Specific Aim 3). Optical instrumentation to noninvasively monitor hydrogels in various vessels and form factors will be designed, built, and tested (Specific Aim 4).


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2021	TEXAS A&M ENGINEERING EXPERIMENT STATION	3124 TAMU	COLLEGE STATION	TX	77843	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	10/19/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $318,172 )
2021	2021	TEXAS A&M ENGINEERING EXPERIMENT STATION	7607 EASTMARK DR	COLLEGE STATION	TX	77840	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	3/19/2021	NON-COMPETING CONTINUATION	$318,172
2021	2021	TEXAS A&M ENGINEERING EXPERIMENT STATION	7607 EASTMARK DR	COLLEGE STATION	TX	77840	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	4	5/27/2021	NON-COMPETING CONTINUATION	$0
														Subtotal = $318,172

Issue Date FY: 2020 ( Subtotal = $325,116 )
2020	2020	TEXAS A&M ENGINEERING EXPERIMENT STATION	7607 EASTMARK DR	COLLEGE STATION	TX	77840	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	1/22/2020	NON-COMPETING CONTINUATION	$325,116
														Subtotal = $325,116

Issue Date FY: 2019 ( Subtotal = $316,815 )
2019	2019	TEXAS A&M ENGINEERING EXPERIMENT STATION	7607 EASTMARK DR	COLLEGE STATION	TX	77840	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	1/17/2019	NON-COMPETING CONTINUATION	$316,815
														Subtotal = $316,815

Issue Date FY: 2018 ( Subtotal = $312,859 )
2018	2018	TEXAS A&M ENGINEERING EXPERIMENT STATION	7607 EASTMARK DR	COLLEGE STATION	TX	77840	BRAZOS	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	4/12/2018	NEW	$312,859
														Subtotal = $312,859

Grand Total All Awards = $1,272,962

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Minimally-Invasive Metabolite Monitoring Systems for in vitro and in vivo Disease Models

Award Number: R01EB024601

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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