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DYNAMIC IMAGE ANALYSIS OF HUMAN EMBRYONIC STEM CELLS TO MONITOR PLURIPOTENCY

Award Number: R01EB006161
ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/15/2007
PERIOD OF PERFORMANCE END DATE: 10/31/2012

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DYNAMIC IMAGE ANALYSIS OF HUMAN EMBRYONIC STEM CELLS TO MONITOR PLURIPOTENCY - DESCRIPTION (provided by applicant): Human embryonic stem cells (hESC) have great potential for cellular therapy because they are pluripotent. Analytical reliable methodologies for the non-destructive non-invasive quality evaluation and assurance for hESC production would be of great benefit to cellular therapy, drug screening and other uses of hESC. We have found that hESC colony texture and border characteristics provide useful features for determining the colony's level of pluripotency (Mangoubi et al., submitted to IEEE Trans. Biomed. Eng.). Cellular characteristics of pluripotent hESC include nuclear hyperdynamics and lack of chromatin condensation. We propose to develop image based texture and border analysis analytical algorithms that would measure 1) the kinetics of whole hESC colony texture and borders and 2) the kinetics of cell nuclei texture. The methodology is to be used as a quality assurance tool for hESC production by measuring the kinetics of single cell nuclei, chromatin dynamics and heterochromatin formation as pluripotent cells differentiate into neuronal lineages. Our objective is to apply and continue to develop our new signal and image processing methodologies for application to hESC biology. These image processing based methods will be specifically used to 1) evaluate the pluripotency of multicell colonies and single cell nuclei, 2) predict the time history of colony fate, and 3) predict the dynamics of cell production. Development of the stem cell non-destructive evaluation methodology would require beyond state of the art analytical algorithms in the following areas: Parametric and non-Parametric classification, efficient variational segmentation and curve evolution methods, innovative border crispness and diffusivity analysis, non-Gaussian subspace learning and detection methods, and multi-resolution hierarchical dynamic models. Our objective is to develop these analytical tools for quantitative measurement of amorphous cellular and subcellular structures, though these innovations will benefit areas of medical imaging ranging from fMRI to tissue engineering. Our aims include: 1) The development of mathematical image processing methods for quantitatively distinguishing the texture and border of pluripotent from differentiated individual stem cells and colonies, 2) the extension of these methods to kinetic images for measuring dynamic changes in stem cell textures and borders, and 3) the development of spatio-temporal dynamic and control models that predict and help maintain the quality of hESC.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2012 ( Subtotal = $0 )
2012	2010	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	5/16/2012	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2010 ( Subtotal = $396,054 )
2010	2010	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	4	5/31/2010	NON-COMPETING CONTINUATION	$396,054
														Subtotal = $396,054

Issue Date FY: 2009 ( Subtotal = $402,553 )
2009	2009	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	3	5/29/2009	NON-COMPETING CONTINUATION	$402,553
														Subtotal = $402,553

Issue Date FY: 2008 ( Subtotal = $558,004 )
2008	2008	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	001	2	7/18/2008	SUPPLEMENT FOR EXPANSION	$151,910
2008	2008	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	2	5/23/2008	NON-COMPETING CONTINUATION	$406,094
														Subtotal = $558,004

Issue Date FY: 2007 ( Subtotal = $394,161 )
2007	2007	Charles Stark Draper Laboratory, Inc.	555 Technology Sq	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Discovery and Applied Research for Technological Innovations to Improve Human Health	000	1	6/4/2007	NEW	$394,161
														Subtotal = $394,161

Grand Total All Awards = $1,750,772

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DYNAMIC IMAGE ANALYSIS OF HUMAN EMBRYONIC STEM CELLS TO MONITOR PLURIPOTENCY

Award Number: R01EB006161

ORGANIZATION: NATIONAL INSTITUTE Of BIOMEDICAL IMAGING & BIOENGINEERING

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/15/2007

PERIOD OF PERFORMANCE END DATE: 10/31/2012

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